EZ Flask – bioreactor flask for easy hybridoma culture
We compared the 1L EZ flasks with gas exchange membrane to our standard method based on T75 flasks. The hybridoma cells were incubated in EZ or T75 flasks, and the antibody was purified using standard methods. Production in the EZ-flask resulted in considerably higher yields of the MAb (about 32 mg/l) than our standard procedure (19.5 mg/l). The procedure with the EZ flask is very convenient, as the hands-on time is very short, allowing an easy production even over longer time periods.
We produced 31 mg for one of the hybridomas and 8 mg for the other one. (difficult clones). We got, in parallel, the same amount of medium (1 L) from normal cell culture flasks but the antibody amount obtained with these was around 5 times lower than with EZ-flask. We like the yield advantage and ease of use of EZ-Flask.
I tested together with my colleagues the EZ flask. As you can deduce from the data we feel very satisfied with your product EZ Flask, especially comparing your product with a similar in vitro static culture device. We will be using this system for R&D. “
We have grown a hybridoma to obtain mAb. And it effectively significantly increases yield. Growing it in the same conditions of culture media and supplements.”
Easy antibody production from hybridomas
Eliminate passaging of multiple flasks
Generate monoclonal antibody at higher concentration
Save valuable incubator space
No agitation required
EZ-Flask (“easy-flask”) is a single-compartment 1 Litre bioreactor flask that allows continuous proliferation of hybridomas thanks to a 200cm2 gas exchange membrane at the base of the flask. EZ-Flask is therefore ideal for in vitro monoclonal antibody production from hybridomas, see below : “how does it work”. Users report that a single EZ-Flask is equivalent to using 40 to 100 T-Flasks. (N.B. not designed for harvesting cells.)
Simple and easy
The simplest way to use this flask bioreactor? Just fill and inoculate. In 2 to 4 weeks of uninterrupted cell growth a good clone can produce up to 150 mg. More than one hybridoma? These flasks are conviently stackable in your incubator. Re-feeding with fresh media is possible to extend the culture.
Economical monoclonal antibody production
The easy procedure and attractive price makes this is a very economical bioreactor when compared to space-inefficient and laborious T-flasks or expensive dual-compartment flask bioreactors that require regular skilful labour.
How does it work?
After inoculation hybridoma cells will settle directly above the gas permeable membrane at the base of the flask. The cells remain well-oxygenated and nourished and will expand without interruption to form a dense layer. Optimal levels of monoclonal antibody secretion are possible under these continuous high cell-density conditions. Passaging is not required.
Extra performance
EZ-Flask works well with serum-free media.
To maximise productivity, select the best media formulation for your hybridoma with this media screening method using G-Rex multiwell plates (with somple 20X scale-up to EZ-Flask). See G-Rex 24 and 6W Plates.
Certain hybridomas may continue to produce monoclonal antibody following the log growth phase, so the best yields can often be obtained simply by leaving the EZ-flask untouched for 30 days after inoculation. On the other hand, re-feeding with fresh media can extend culture time and total yield per flask. According to user feedback, a single EZ-Flask should produce the same amount of mAb as 40 to 100 T-175 Flasks.
EZ Flask bioreactor flask works by oxygenating the cells from below. This is achieved by a base gas exchange membrane. Inoculated cells gravitate to the base and, staying fully oxygenated, proliferate for up to 25 days, doubling many times in 1 Litre of media.
EZ-Flask is designed for growing hybridomas for producing monoclonal antibodies. It is not designed for cell harvesting applications.
If you have never used EZ-Flask before we recommend to grow 50 million cells in T-Flask and use these to inoculate 1 Litre of media. Please check out the two culture procedures we suggest.
The easiest procedure is to fill the flask with 1 Litre of your usual hybridoma culture media, inoculate hybridomas (between 25 and 50 million), then wait for 30 days before harvesting 1L of monoclonal antibody. Do not disturb the flask during the culture period. You might want to try the alternative procedure if you have low starting cell number, serum-free media or a difficult to grow clone.
The alternative procedure for using EZ-Flask is to fill with 250ml of your usual culture media, ideally with some T-Flask conditioned media, then inoculate 10 to 25 million cells. Wait 2 days then add 250 ml. Wait another 3 or 4 days then add 500 ml to take the total volume up to 1 Litre. On day 30 harvest 1 Litre mAb.
| Cat.# | Description | Volume ml | Membrane area cm2 |
|---|---|---|---|
| KDW0010 | EZ Flask | 1000 | 200 |
Only available to customers that have purchased this product in the past.
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