EZ Flask – bioreactor flask for easy hybridoma culture
Only available to customers that have purchased this product in the past.
Easily grow billions of hybridomas in a single flask
Eliminate passaging of multiple flasks
Generate monoclonal antibody at higher concentration
Save valuable incubator space
EZ-Flask (“easy-flask”) is a single-compartment 1 Litre bioreactor flask that allows continuous proliferation of hybridomas thanks to a 200cm2 gas exchange membrane at the base of the flask. EZ-Flask is therefore ideal for in vitro monoclonal antibody production from hybridomas, see below : “how does it work”. Users reprort that a single EZ-Flask is equivalent to using 40 to 100 T-Flasks.
Simple and easy
The simplest way to use this flask bioreactor? Just fill and inoculate. In 2 to 4 weeks of uninterrupted cell growth a good clone can produce up to 150 mg. More than one hybridoma? These flasks are conviently stackable in your incubator. Re-feeding with fresh media is possible to extend the culture.
Economical monoclonal antibody production
The easy procedure and attractive price makes this is a very economical bioreactor when compared to space-inefficient and laborious T-flasks or expensive dual-compartment flask bioreactors that require regular skilful labour.
How does it work?
After inoculation hybridoma cells will settle directly above the gas permeable membrane at the base of the flask. The cells remain well-oxygenated and nourished and will expand without interruption to form a dense layer. Optimal levels of monoclonal antibody secretion are possible under these continuous high cell-density conditions. Passaging is not required.
Extra performance
Certain hybridomas may continue to produce monoclonal antibody following the log growth phase, so the best yields can often be obtained simply by leaving the flask untouched for 30 days after inoculation. On the other hand, re-feeding with fresh media can extend culture time and total yield per flask. According to user feedback, a single EZ-Flask should produce the same amount of mAb as 40 to 100 T-175 Flasks.
EZ-Flask has been used successfully with serum-free media such as ISF-1.
We have tested the EZ-Flask with two hybridomas in serum free medium. The initial load was of the order of 10^7, and cells grew well. We initially added 250 ml of medium and added 250 ml every week up to 1L using the protocol #2 you gave us. We purified the antibodies and we obtained an amount of 31 mg for one of the hybridomas and 8 mg for the other one. (difficult clones). We got, in parallel, the same amount of medium (1 L) from normal cell culture flasks but the antibody amount obtained was around 5 times lower than with EZ-flask.
” I tested together with my colleagues the EZ flask. As you can deduce from the data we feel very satisfied with your product EZ Flask, especially comparing your product with a similar in vitro static culture device. We will be using this system for R&D. For larger scale productions of IgG we will switch to Hollow Fiber“
” We have grown a hybridoma to obtain mAb. And it effectively significantly increases yield. Growing it in the same conditions of culture media and supplements.”
Other suspension cell types and organoids
All types of suspension cells can be expanded to high numbers without passaging in the EZ-Flask, e.g. CHO, Jurkat, B-cells, THP1. EZ-Flask and G-Rex Plates are particularly well suited for the development of suspension CHO cell lines.
Recently the same gas membrane exchange technology has been shown to allow the long-term maintenance of hiPSC-derived organoids. See : “Self-organized emergence of hyaline cartilage in hiPSC-dervied multi-tissue organoids” .
Use G-Rex® Plates to optimize culture conditions for EZ-Flask
Each well of a G-Rex Plate can be used as a scaled-down version of an EZ Flask.
Each well essentially behaves like a “mini bioreactor”. These plates therefore provide a fast and convenient way to optimize parameters such as seeding density (cells/cm2 membrane), media, duration, etc.
G-Rex Plates are very convenient for developing monoclonal antibodies and for hybridoma clone selection.
Discover G-Rex Plates for cell expansion without passaging.
| Cat.# | Description | Volume ml | Membrane area cm2 |
|---|---|---|---|
| KDW0010 | EZ Flask | 1000 | 200 |
| KDW0026 | G-Rex 6 | 35/well | 10/well |
| KDW0026 | G-Rex 24 | 7/well | 2/well |
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