EZ Flask – bioreactor flask for easy hybridoma culture

  • Easily grow billions of hybridomas in a single flask

  • Eliminate passaging of multiple flasks

  • Generate monoclonal antibody at higher concentration

  • Save valuable incubator space

EZ-Flask (“easy-flask”) is a single-compartment 1 Litre bioreactor flask that allows continuous proliferation of hybridomas and other suspension cells thanks to a 200cm2 gas exchange membrane at the base of the flask. EZ-Flask is ideal for in vitro monoclonal antibody production from hybridomas, see below : “how does it work”.  Users reprort that a single EZ-Flask can produce the same as 40 to 100 T-Flasks.

Simple and easy 

The simplest way to use this flask bioreactor? Just fill and inoculate. In 2 to 4 weeks of uninterrupted cell growth a good clone can produce up to 150 mg. More than one hybridoma? These flasks are conviently stackable in your incubator. Re-feeding with fresh media is possible to extend the culture.

Economical monoclonal antibody production

The easy procedure and attractive price makes this is a very economical bioreactor when compared to space-inefficient and laborious T-flasks or expensive dual-compartment flask bioreactors that require regular skilful labour.

How does it work?

After inoculation hybridoma cells will settle directly above the gas permeable membrane at the base of the flask. The cells remain well-oxygenated and nourished and will expand without interruption to form a dense layer. Optimal levels of monoclonal antibody secretion are possible under these continuous high cell-density conditions. Passaging is not required.

Extra performance

Certain hybridomas may continue to produce monoclonal antibody following the log growth phase, so the best yields can often be obtained simply by leaving the flask untouched for 30 days after inoculation. On the other hand, re-feeding with fresh media can extend culture time and total yield per flask. According to user feedback, a single EZ-Flask should produce the same amount of mAb as 40 to 100 T-175 Flasks.

EZ-Flask has been used successfully with serum-free media such as ISF-1.

We have tested the EZ-Flask with two hybridomas in serum free medium. The initial load was of the order of 10^7, and cells grew well. We initially added 250 ml of medium and added 250 ml every week up to 1L using the protocol #2 you gave us. We purified the antibodies and we obtained an amount of 31 mg for one of the hybridomas and 8 mg for the other one. (difficult clones). We got, in parallel, the same amount of medium (1 L) from normal cell culture flasks but the antibody amount obtained was around 5 times lower than with EZ-flask.

mAb producer in Spain

” I tested together with my colleagues the EZ flask. As you can deduce from the data we feel very satisfied with your product EZ Flask, especially comparing your product with a similar in vitro static culture device. We will be using this system for R&D. For larger scale productions of IgG we will switch to Hollow Fiber

Cristina Scozzesi, Dia.Pro. Srl

” We have grown a hybridoma to obtain mAb. And it effectively significantly increases yield. Growing it in the same conditions of culture media and supplements.”

Isabel Garcia, EUROFINS

Other suspension cell types and organoids

All types of suspension cells can be expanded to high numbers without passaging in the EZ-Flask, e.g. CHO, Jurkat, B-cells, THP1. EZ-Flask and G-Rex Plates are particularly well suited for the development of suspension CHO cell lines.

Recently the same gas membrane exchange technology has been shown to allow the long-term maintenance of hiPSC-derived organoids. See : “Self-organized emergence of hyaline cartilage in hiPSC-dervied multi-tissue organoids” .

Use G-Rex® Plates to optimize culture conditions for EZ-Flask

Each well of a G-Rex Plate can be used as a scaled-down version of an EZ Flask.

Each well essentially behaves like a “mini bioreactor”. These plates therefore provide a fast and convenient way to optimize parameters such as seeding density (cells/cm2 membrane), media, duration, etc.

G-Rex Plates are very convenient for developing monoclonal antibodies and for hybridoma clone selection.

Discover G-Rex Plates for cell expansion without passaging.

G-Rex plates
Cat.#DescriptionVolume mlMembrane area cm2
KDW0010EZ Flask1000200
KDW0026G-Rex 635/well10/well
KDW0026G-Rex 247/well2/well

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