EZ Flask – the easiest way to culture hybridomas
1 Litre of monoclonal antibody with NO PASSAGING required
Much higher mAb titres than with conventional methods
Simple protocol avoids user interventions and reduces contamination risk
The EZ-Flask (“easy-flask”) ensures rapid and continuous proliferation of hybridomas and other suspension cells without the need for passaging. This new cell culture flask is ideal for in vitro monoclonal antibody production from hybridomas. Very high cell numbers and therefore higher yields are obtained with EZ-Flask thanks to a 200cm2 base gas exchange membrane (see “how does it work”, below). No passaging or time-consuming routine manipulations are needed – just fill with media, inoculate, wait a couple of weeks and then harvest 1 litre of high titre mAb. A single EZ-Flask can be equivalent to between 20 and 100 x T175 flasks.
Simple and easy – high yields with no passaging required
Just fill and inoculate. No need for cell passaging. In 2 to 4 weeks the process is complete and with a good clone harvest yields can be up to 150 mg /Litre. Flasks are stackable in the incubator.
Economical monoclonal antibody production
The simple design concept makes this is a very economical bioreactor especially when compared to laborious flasks where cell passaging is needed. It can also save a lot of valuable incubator space that might otherwise be wasted on T-Flasks.
How does it work?
After inoculation hybridomas settle directly above the gas permeable membrane at the base of the cell culture flask. Well-oxygenated and nourished at all times, the cells will proliferate without interruption to form a dense layer and are much more productive than in T-flasks or roller bottles. Stressed hybridomas, that increase their monoclonal antibody production following the log growth phase can be fully exploited in this system. A single EZ-Flask can replace even up to 100 T-Flasks and works with serum-containing DMEM or serum-free ISF-1 media.
We have tested the EZ-Flask with two hybridomas in serum free medium. The initial load was of the order of 10^7, and cells grew well. We initially added 250 ml of medium and added 250 ml every week up to 1L using the protocol #2 you gave us. We purified the antibodies and we obtained an amount of 31 mg for one of the hybridomas and 8 mg for the other one. (difficult clones). We got, in parallel, the same amount of medium (1 L) from normal cell culture flasks but the antibody amount obtained was around 5 times lower than with EZ-flask.
” I tested together with my colleagues the EZ flask. As you can deduce from the data we feel very satisfied with your product EZ Flask, especially comparing your product with a similar in vitro static culture device. We will be using this system for R&D. For larger scale productions of IgG we will switch to Hollow Fiber“
” We have grown a hybridoma to obtain mAb. And it effectively significantly increases yield. Growing it in the same conditions of culture media and supplements.”
Other suspension cell types and organoids
All types of suspension cells can be expanded to high numbers without passaging in the EZ-Flask, e.g. CHO, Jurkat, B-cells, THP1. EZ-Flask and G-Rex Plates are particularly well suited for the development of suspension CHO cell lines.
Recently the same gas membrane exchange technology has been shown to allow the long-term maintenance of hiPSC-derived organoids. See : “Self-organized emergence of hyaline cartilage in hiPSC-dervied multi-tissue organoids” .
Use G-Rex® Plates to optimize culture conditions for EZ-Flask
Each well of a G-Rex Plate can be used as a scaled-down version of an EZ Flask.
Each well essentially behaves like a “mini bioreactor”. These plates therefore provide a fast and convenient way to optimize parameters such as seeding density (cells/cm2 membrane), media, duration, etc.
G-Rex Plates are very convenient for developing monoclonal antibodies and for hybridoma clone selection.
Discover G-Rex Plates for cell expansion without passaging.
| Cat.# | Description | Volume ml | Membrane area cm2 |
|---|---|---|---|
| KDW0010 | EZ Flask | 1000 | 200 |
| KDW0026 | G-Rex 6 | 35/well | 10/well |
| KDW0026 | G-Rex 24 | 7/well | 2/well |
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