EZ Flask – monoclonal antibody production flask

Ez-Flask for in vitro monoclonal antibody production
  • No passaging

  • High-titre mAb batch production

  • Works with hybridomas and all suspension cells

  • 2 simple protocols for productions up to 30 days

The EZ-Flask (“easy-flask”) is a new cell culture flask tailored for very easy in vitro monoclonal antibody production from hybridomas. Higher yields are obtained from EZ-Flask thanks to a gas exchange membrane. The design also means that no passaging or tricky routine manipulations are needed – just fill with media, inoculate, wait a couple of weeks and then harvest 1 litre of high titre mAb.

1) Simple and easy – high yields with no passaging required !

Just fill and inoculate. No need for cell passaging. In 2 to 4 weeks the process is complete and with a good clone you can harvest yields up to 150 mg in 1 Litre.

Inoculation of EZ-Flask

2) Economical monoclonal antibody production

The simple design concept makes this is a very economical bioreactor especially when compared to laborious flasks where cell passaging is needed. It can also save a lot of valuable incubator space that might otherwise be wasted on T-Flasks. In terms of monoclonal antibody productivity, 1 EZ-Flask is equivalent to 20 – 100x T-175 flasks depending on the clone. Some users report yields of up to 150mg of mAb after 30 days of culture.

3) How does it work?

After inoculation hybridomas will quickly proliferate to form a dense layer above the gas permeable membrane at the base of the cell culture flask.

Very well-oxygenated and nourished at all times, the cells are thus much more productive than in T-flasks or roller bottles.

Stressed hybridomas, that increase their monoclonal antibody production following the log growth phase can be fully exploited in this system.

 

We have tested the EZ-Flask with two hybridomas in serum free medium. The initial load was of the order of 10^7, and cells grew well. We initially added 250 ml of medium and added 250 ml every week up to 1L using the protocol #2 you gave us. We purified the antibodies and we obtained an amount of 31 mg for one of the hybridomas and 8 mg for the other one. (difficult clones)

However, we got in parallel the same amount of medium (1 L) from normal cell culture flasks and the antibody amount obtained was around 5 times lower than with EZ-flask.

We would have needed more than a 100 T-Flasks to obtain the same amount we did in EZ-Flask.

mAb producer in Spain

” I tested together with my colleagues the EZ flask. As you can deduce from the data we feel very satisfied with your product EZ Flask, especially comparing your product with a similar in vitro static culture device. We will be using this system for R&D. For larger scale productions of IgG we will switch to Hollow Fiber

Cristina Scozzesi, Dia.Pro. Srl

” We have grown a hybridoma to obtain mAb. And it effectively significantly increases yield. Growing it in the same conditions of culture media and supplements.”

Isabel Garcia, EUROFINS

Other suspension cell types

All types of suspension cells can be expanded to high numbers without passaging in the EZ-Flask, e.g. CHO, Jurkat, B-cells, THP1.

EZ-Flask and G-Rex Plates are particularly well suited for the development of suspension CHO cell lines.

 

Use G-Rex® Plates to optimize culture conditions for EZ-Flask

Each well of a G-Rex Plate can be used as a scaled-down version of an EZ Flask.

Each well essentially behaves like a “mini bioreactor”. These plates therefore provide a fast and convenient way to optimize parameters such as seeding density (cells/cm2 membrane), media, duration, etc.

G-Rex Plates are very convenient for developing monoclonal antibodies and for hybridoma clone selection.

Discover G-Rex Plates for cell expansion without passaging.

G-Rex plates

Compare EZ-Flask, G-Rex and FibreCell Systems Hollow Fibre for monoclonal antibody production.

Cat.#DescriptionVolume mlMembrane area cm2
KDW0010EZ Flask1000200
KDW0026G-Rex 635/well10/well
KDW0026G-Rex 247/well2/well

EZ Flask is manufactured by Wilson Wolf (USA).
Distributed by KDBIO in all EU countries + CH, NO, UK

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