Simple and easy – high yields with no passaging required
Just fill and inoculate. No need for cell passaging. In 2 to 4 weeks the process is complete and with a good clone harvest yields can be up to 150 mg /Litre. Flasks are stackable in the incubator.
Economical monoclonal antibody production
The simple design concept makes this is a very economical bioreactor especially when compared to laborious flasks where cell passaging is needed. It can also save a lot of valuable incubator space that might otherwise be wasted on T-Flasks.
How does it work?
After inoculation hybridomas settle directly above the gas permeable membrane at the base of the cell culture flask. Well-oxygenated and nourished at all times, the cells will proliferate without interruption to form a dense layer and are much more productive than in T-flasks or roller bottles. Stressed hybridomas, that increase their monoclonal antibody production following the log growth phase can be fully exploited in this system. A single EZ-Flask can replace even up to 100 T-Flasks and works with serum-containing DMEM or serum-free ISF-1 media.
Other suspension cell types and organoids
All types of suspension cells can be expanded to high numbers without passaging in the EZ-Flask, e.g. CHO, Jurkat, B-cells, THP1. EZ-Flask and G-Rex Plates are particularly well suited for the development of suspension CHO cell lines.
Recently the same gas membrane exchange technology has been shown to allow the long-term maintenance of hiPSC-derived organoids. See : “Self-organized emergence of hyaline cartilage in hiPSC-dervied multi-tissue organoids” .
Use G-Rex® Plates to optimize culture conditions for EZ-Flask
Each well of a G-Rex Plate can be used as a scaled-down version of an EZ Flask.
Each well essentially behaves like a “mini bioreactor”. These plates therefore provide a fast and convenient way to optimize parameters such as seeding density (cells/cm2 membrane), media, duration, etc.
G-Rex Plates are very convenient for developing monoclonal antibodies and for hybridoma clone selection.
Discover G-Rex Plates for cell expansion without passaging.