Home>admin

About admin

This author has not yet filled in any details.
So far admin has created 57 blog entries.

piggyBac transposon-based expression system in combination with FiberCell hollow fibre bioreactor enables remarkable levels of production of HexM expression

100 x increase in protein yield accelerates ERT preclinical research Preclinical studies with potential therapeutic recombinant proteins can be hampered by difficulties with expression and scale-up in the laboratory. A group at the Department of Manitoba, Canada, used the piggyBac expression system in conjunction with the compact FiberCell hollow fibre bioreactor to generate >500mg [...]

By |2021-12-26T17:34:09+00:00December 26th, 2021|News|0 Comments

Improving the Drug Development Pipeline for Mycobacteria

[Review]   Modelling Antibiotic Exposure in the Hollow Fibre Infection Model Maitra, A.; Solanki, P.;Sadouki, Z.; McHugh, T.D.;Kloprogge, F. Improving the Drug Development Pipeline for Mycobacteria: Modelling Antibiotic Exposure in the Hollow Fibre Infection Model. Antibiotics 2021, 10, 1515. https://doi.org/10.3390/antibiotics10121515 Mycobacterial infections are difficult to treat, requiring a combination of drugs and lengthy treatment times, [...]

By |2021-12-20T10:28:45+00:00December 20th, 2021|HFIM News, News|0 Comments

Protocol for continuous culture of mesenchymal stem cells at high density in a hollow fibre bioreactor to produce concentrated exosomes

For laboratories wishing to produce large amounts of extracellular vesicles (exosomes) from MSCs using a hollow fibre bioreactor, FiberCell Systems has now published a preliminary protocol based on feedback from users in Europe and USA. If you need to quickly generate concentrated and pure EVs from MSCs this document is definitely a "must read". [...]

By |2021-11-02T13:45:24+00:00November 2nd, 2021|News|0 Comments

Standard therapy of MAC pulmonary disease shows limited efficacy in HFIM

Standard therapy of Mycobacterium avium complex pulmonary disease shows limited efficacy in an open source hollow fibre system that simulates human plasma and epithelial lining fluid pharmacokinetics : Ruth MM. et al.; Clinical Microbiology and Infection 2021 [abstract] Note : Supplementary information provided with this publication about the practical aspects of the set-up is helpful [...]

By |2021-10-17T16:28:08+00:00October 17th, 2021|HFIM News|0 Comments

Media advice for celline flask production of monoclonal antibodies

High density celline flask culture of hybridomas is made more efficient by completely eliminating serum from both compartments. A serum replacement called CDM-HD or "Chemically-Defined Media for High Density Culture" is specifically designed for high density cultures and is supplied in Europe exclusively through KDBIO. It's important to note that this simplified media only [...]

By |2021-10-03T11:39:05+00:00October 3rd, 2021|News|0 Comments

Meet KDBIO at ECI2021 Virtual Exhibition

KDBIO Virtual Exhibition Stand at ECI2021 IN YOUR OWN LAB - produce significant amounts of monoclonal antibodies, recombinant proteins or extracellular vesicles using a FiberCell hollow fibre bioreactor. See also EZ-Flask for growing high numbers of hybridomas without passaging.

By |2021-09-02T05:30:46+00:00September 1st, 2021|Events|0 Comments

Development of monoclonal antibody using G-Rex Plates

In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines. Following the successful fusion, clone selection and weaning from media supplements ten candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well). [...]

By |2021-10-29T07:13:41+00:00August 19th, 2021|News|0 Comments

EV Core Poster

Comparison of Production and Activity of Amniotic Fluid Stem Cell Extracellular Vesicles from 3D Hollow Fiber Bioreactor and 2D culture. ISEV2020 Poster Laura Perin et al. ;Introduction: EV clinical translation is limited by scale-up of EVs production. Hollow fiber bioreactors (HFBR) support culture of large numbers of cells at high densities. [...]

By |2021-08-12T08:59:16+00:00August 12th, 2021|News|0 Comments

Scaling up production of Netrin-1 for structural biology, functional and biophysical studies

Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor Applied Microbiology and Biotechnology August 2021 https://doi.org/10.1007/s00253-021-11438-0 Aniel Moya-Torres et al. Key Points: Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 [...]

By |2021-09-15T10:26:39+00:00August 11th, 2021|News|0 Comments

U-Cytech Switches to FiberCell Hollow Fiber cell culture for mAb production

U-CyTech biosciences switches from roller bottles to FiberCell Systems Hollow Fibre Bioreactor U-CyTech biosciences is based in Utrecht, the Netherlands, and produces a wide range of innovative cytokine ELISA, ELISPOT and Fluorospot systems (human, monkey, rodent). Productions of monoclonal antibody with hybridomas are now performed at U-CyTech’s Utrecht laboratory using the FiberCell Hollow Fibre Bioreactor [...]

By |2021-10-06T12:53:05+00:00June 15th, 2021|News|0 Comments
Go to Top