KDBIO Virtual Exhibition Stand at ECI2021 IN YOUR OWN LAB - produce significant amounts of monoclonal antibodies, recombinant proteins or extracellular vesicles using a FiberCell hollow fibre bioreactor. See also EZ-Flask for growing high numbers of hybridomas without passaging.
In this example : monoclonal antibody development results for 10 newly developed monoclonal cell lines. Following successful fusion, clone selection and weaning from media supplements ten candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well). Cell culture supernatant was purified with [...]
Comparison of Production and Activity of Amniotic Fluid Stem Cell Extracellular Vesicles from 3D Hollow Fiber Bioreactor and 2D culture. ISEV2020 Poster Laura Perin et al. ;Introduction: EV clinical translation is limited by scale-up of EVs production. Hollow fiber bioreactors (HFBR) support culture of large numbers of cells at high densities. [...]
Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor Applied Microbiology and Biotechnology August 2021 https://doi.org/10.1007/s00253-021-11438-0 Aniel Moya-Torres et al. Key Points: Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 [...]
U-CyTech biosciences switches from roller bottles to FiberCell Systems Hollow Fibre Bioreactor U-CyTech biosciences is based in Utrecht, the Netherlands, and produces a wide range of innovative cytokine ELISA, ELISPOT and Fluorospot systems (human, monkey, rodent). Productions of monoclonal antibody with hybridomas are now performed at U-CyTech’s Utrecht laboratory using the FiberCell Hollow Fibre [...]
Monoclonal antibody production (hybridoma) in different systems Monoclonal antibody production can be achieved using various culture systems. Depending on the yield you are looking for you might need a different type of system. KDBIO offers 3 scale-up cell culture systems from small batch quantities up to continuous gram production quantities. Below you will find a [...]
ISEV2021 provides a great opportunity to discuss 3D CM-EVs production Large amounts of EVs can be obtained from practically any type of cell, including mesenchymal stems cells, maintained over a few weeks at high density in a FiberCell Hollow Fibre Bioreactor. A medium size 20ml hollow fibre cartridge (C2011) offers 4000cm2 of porous fibre surface [...]
New YouTube Channel - Hollow fibre bioreactor technology and applications KDBIO has launched a Youtube channel with short videos to explain hollow fibre bioreactor technology and the advantages this offers for various applications. KDBIO YouTube Channel
Access lists of relevant references / Extracellular vesicles production / Monoclonal antibodies production / Recombinant proteins production / Secretome from 3D cultures
CDM-HD : chemically-defined media for high density culture Once cells are growing at high density in a FiberCell bioreactor it is generally very easy to switch over to CDM-HD (DMEM/10%CMD-HD) which is ideal for generating cell-secreted products such as monoclonal antibodies or extracellular vesicles.