Osteosarcoma-Derived Extracellular Vesicles Induce Lung Fibroblast Reprogramming Alek Mazumdar et al., Dept. of Orthopedics, Balgrist University Hospital, Zurich, Switzerland; Int. J. Mol. Sci. 2020, 21, 5451; doi:10.3390/ijms21155451 "....our findings provide novel insights into the functional relevance of EV-mediated communication between osteosarcoma cells and normal pulmonary fibroblasts. We deliver strong evidence that osteosarcoma cell-derived EVs [...]

Which culture method is best to generate reagents with native biological specificity? Cell culture methods vary a lot with respect to their ability to generate secreted proteins and antibodies that possess true biological specificity. Culture methodology is therefore a critical consideration for a manufacturer of serological test kits where excellent specificity is a priority. [...]

Here's how hollow fibre culture could be useful for Coronavirus research. FIRSTLY - Efficient routine production (100mg to grams) of recombinant viral proteins and monoclonal antibodies for TEST KITS to assess the immune response to SARS-CoV-2 in the population. Also, mAbs for the quantification and characterisation of VACCINES prior to lot release : [...]

Understand (almost) all you need to know about hollow fiber bioreactor technology in under a minute. Extracellular Vesicles / Exosomes Monoclonal Antibodies Recombinant Proteins 3D culture in vitro models FiberCell Systems Hollow Fibre Bioreactor - keep up to 10^11 cells in continuous culture

Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity Abstract: Animal and clinical studies have shown that mesenchymal stem cells (MSCs) play an important role in cartilage repair. The therapeutic effect of mesenchymal stem cells based therapies has been increasingly demonstrated to exosome-mediated paracrine [...]

The Effect of Drug Combinations on the Kinetics of the Emergence of Antibiotic Resistance in E. coli CFT073 Using the In Vitro Hollow Fiber Infection Model Abstract: Antibiotic resistance is one of the major threats to public health today. To address this problem requires an urgent comprehensive approach. Strategic and multi-targeted combination therapy [...]

In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds Abstract: Hollow fiber technology is a powerful tool for the culture of difficult-to-grow cells. Cryptosporidium parvum has a multistage sexual and asexual life cycle that has proved difficult to culture by conventional in vitro [...]