U-CyTech biosciences switches from roller bottles to FiberCell Systems Hollow Fibre Bioreactor U-CyTech biosciences is based in Utrecht, the Netherlands, and produces a wide range of innovative cytokine ELISA, ELISPOT and Fluorospot systems (human, monkey, rodent). Productions of monoclonal antibody with hybridomas are now performed at U-CyTech’s Utrecht laboratory using the FiberCell Hollow Fibre [...]
mAb production (hybridoma) in different systems G-Rex 6 Well Plate EZ-Flask FiberCell C2011 Batch or Continuous Batch Batch Continuous Harvest volume 35 ml / well 1 Litre 20ml, 3X per week Duration of production 15 to 30 days 15 - 30 days Typically 2 to 3 months Production range (mg) 0.15 to 7.5 [...]
ISEV2021 provides a great opportunity to discuss 3D CM-EVs production Large amounts of EVs can be obtained from practically any type of cell, including mesenchymal stems cells, maintained over a few weeks at high density in a FiberCell Hollow Fibre Bioreactor. A medium size 20ml hollow fibre cartridge (C2011) offers 4000cm2 of porous fibre surface [...]
New YouTube Channel - Hollow fibre bioreactor technology and applications KDBIO has launched a Youtube channel with short videos to explain hollow fibre bioreactor technology and the advantages this offers for various applications. KDBIO YouTube Channel
Access lists of relevant references / Extracellular vesicles production / Monoclonal antibodies production / Recombinant proteins production / Secretome from 3D cultures
CDM-HD : chemically-defined media for high density culture Once cells are growing at high density in a FiberCell bioreactor it is generally very easy to switch over to CDM-HD (DMEM/10%CMD-HD) which is ideal for generating cell-secreted products such as monoclonal antibodies or extracellular vesicles.
Osteosarcoma-Derived Extracellular Vesicles Induce Lung Fibroblast Reprogramming Alek Mazumdar et al., Dept. of Orthopedics, Balgrist University Hospital, Zurich, Switzerland; Int. J. Mol. Sci. 2020, 21, 5451; doi:10.3390/ijms21155451 "....our findings provide novel insights into the functional relevance of EV-mediated communication between osteosarcoma cells and normal pulmonary fibroblasts. We deliver strong evidence that osteosarcoma cell-derived EVs [...]
Which culture method is best to generate reagents with native biological specificity? Cell culture methods vary a lot with respect to their ability to generate secreted proteins and antibodies that possess true biological specificity. Culture methodology is therefore a critical consideration for a manufacturer of serological test kits where excellent specificity is a priority. [...]
Here's how hollow fibre culture could be useful for Coronavirus research. FIRSTLY - Efficient routine production (100mg to grams) of recombinant viral proteins and monoclonal antibodies for TEST KITS to assess the immune response to SARS-CoV-2 in the population. Also, mAbs for the quantification and characterisation of VACCINES prior to lot release : [...]
Understand (almost) all you need to know about hollow fiber bioreactor technology in under a minute. Extracellular Vesicles / Exosomes Monoclonal Antibodies Recombinant Proteins 3D culture in vitro models FiberCell Systems Hollow Fibre Bioreactor - keep up to 10^11 cells in continuous culture