BioBUZZ Article about hollow fibre culture of cells "If you’ve worked in cell culture, you’ve surely had experience dealing with tissue culture flasks. These plastic vessels are the staple of cell culture work, but they definitely have their share of shortcomings. Having to transfer your cells to gradually larger flasks to scale up. Carefully [...]
Long term culture of A549 cells at high density A compact laboratory hollow fibre culture system provides a convenient way to culture a very large number of cells continuously at high density (> 1e8 cells per ml). This is why, for an R&D laboratory working with incubators, hollow fibre culture is an ideal method [...]
Osteosarcoma-Derived Extracellular Vesicles Induce Lung Fibroblast Reprogramming Alek Mazumdar et al., Dept. of Orthopedics, Balgrist University Hospital, Zurich, Switzerland; Int. J. Mol. Sci. 2020, 21, 5451; doi:10.3390/ijms21155451 "....our findings provide novel insights into the functional relevance of EV-mediated communication between osteosarcoma cells and normal pulmonary fibroblasts. We deliver strong evidence that osteosarcoma cell-derived EVs [...]
Which culture method is best to generate reagents with native biological specificity? Cell culture methods vary a lot with respect to their ability to generate secreted proteins and antibodies that possess true biological specificity. Culture methodology is therefore a critical consideration for a manufacturer of serological test kits where excellent specificity is a priority. [...]
Here's how hollow fibre culture could be useful for Coronavirus research. FIRSTLY - Efficient routine production (100mg to grams) of recombinant viral proteins and monoclonal antibodies for TEST KITS to assess the immune response to SARS-CoV-2 in the population. Also, mAbs for the quantification and characterisation of VACCINES prior to lot release : [...]
Understand (almost) all you need to know about hollow fiber bioreactor technology in under a minute. Extracellular Vesicles / Exosomes Monoclonal Antibodies Recombinant Proteins 3D culture in vitro models FiberCell Systems Hollow Fibre Bioreactor - keep up to 10^11 cells in continuous culture
Meet KDBIO at the IGLD/GSEV conference in Frankfurt to discuss how various laboratories worldwide are discovering how to ramp-up EV production capacity from MSCs and other types of cells using hollow fibre bioreactor technology. https://www.igld.de/
Meet KDBIO at the 4th Annual MarketsandMarkets Next Gen Immuno-Oncology Congress to discuss production of "difficult-to-express" mAbs and other proteins using continuous high density cell culture for more "in-vivo like" culture conditions. https://events.marketsandmarkets.com/4th-annual-marketsandmarkets-next-gen-immuno-oncology-congress/
ECCMID 2020, Paris Come to the KDBIO booth at ECCMID to discuss the hollow fibre system for: 1) precise in-vitro modelling of human PK and analysis of pharmacodynamics as well as following the development of resistance over time and in response to different drug combination treatments 2) Efficient production of mAb and rec. proteins [...]
Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity Abstract: Animal and clinical studies have shown that mesenchymal stem cells (MSCs) play an important role in cartilage repair. The therapeutic effect of mesenchymal stem cells based therapies has been increasingly demonstrated to exosome-mediated paracrine [...]