Mass production of pseudo-islets

Produce standardized and scalable Pseudo-Islets with uniform morphology from different species and beta cell lines

Pseudo-islet production criteria

When generating pseudo-islets different cell-assembly approaches can be considered before selecting a standard method. The chosen method should meet user criteria such as spheroid size, morphology, and consistency as well as the time, labour and amount of material required for producing the target number of spheroids. For cell therapy R&D applications, including beta-cell replacement for type 1 diabetes, thousands to millions of uniform spheroids are needed. In this case the SP5D 6-Well plate is perfectly adapted:

  • Scalable – 20 184 pseudo-islets produced per plate
  • Easy maintenance, harvesting and control of spheroid size
  • Automation-friendly plate
  • Imaging facilitated by central position in microwell

Examples of islets

The following have been successfully cultivated in the SP5D plate:

  • Primary human, pig and rodent islet cells
  • Beta cell lines (EndoC-βH1, MIN-6)

Product Page : SP5D Well Plates

References:

Charles-Henri Wassmer, Ekaterine Berishvili, et al., Cell Transplantation Journal,
2020. https://doi.org/10.1177/0963689720937292

Undine Schubert, Barbara Ludwig, et al., IPITA Congress Abstract, 2021. https://journals.lww.com/transplantjournal/Fulltext/2021/12001/402_8__Neonatal_porcine_Pseudo_Islets___A.48.aspx

Stefan Bornstein, Constanze Hantel, et al., Cell Death & Disease Journal, 2022. https://www.nature.com/articles/s41419-022-05096-x

Honarpisheh M, Seissler J, et al.,Transpl Int. Journal, 2022. https://www.frontierspartnerships.org/articles/10.3389/ti.2022.10697/full

Simple procedure for all SP5D plate types – 6 well version shown below

PREPARE functional wells with 1 ml medium

ADD CELLS – 1 ml of single cell suspension

CULTIVATE spheroids in incubator

Product Page : SP5D 6-Well Plate

References:

Lisa-Marie Philipp, Pirsch Matthias, et al., Synentec Application Note : https://www.sp5d.com/cm/wp-content/uploads/SYNENTECkugelmeiers_sphericalplate_5d_FINAL.pdf

Nishant S.Kulkarni, Vivek Gupta, et al., Materials Science and Engineering
Journal, 2021 : https://doi.org/10.1016/j.msec.2021.111891

Laura Schreyer, Susanne Muehlich, et al., Cancers Journal, 2021 : https://doi.org/10.3390/cancers13215373

Tristen V. Tellman, Mary C. Farach-Carson, et al., International Journal of Molecular Sciences, 2021 : https://doi.org/10.3390/ijms22063218