How does EZ-Flask work?
At the base of the EZ-Flask is a gas exchange membrane with a surface area of 200cm2. After inoculation, suspension cells will gravitate to the base of the flask and settle above the gas exchange membrane. Always close to the gas interface, the cells will expand exponentially, without interruption, in a 1 Litre volume of media. With conventional flasks the height of media above the cells restricts the diffusion of oxygen meaning that the amount of media is always very limited. Media height is not a limitation when the flask is equipped with a base gas exchange membrane and so the EZ-Flask can be filled with 1 Litre of media despite its small footprint in the incubator.
EZ-Flask creates a high-density culture environment
After only a few days under the novel culture conditions described above, a dense carpet of suspension cells will be growing at the base of flask of the EZ-Flask. These auto-stimulatory high-density culture conditions cannot be achieved in T-Flasks or multilayer flasks and yet may be the decisive factor for the efficient cultivation of difficult-to-grow cells.
EZ-Flask means less work and stress
The 1 Litre EZ-Flask eliminates the need for passaging large numbers of flasks and tiresome weekend culture work. It also avoids annoying other users of the incubator with multiple space-consuming T-Flasks.
The EZ-Flask (Cat.# KDW0010) with 1 litre volume and 200cm2 base gas exchange membrane is distributed in Europe by KDBIO.
Please don’t hesitate to send any questions about using this simple culture device : [email protected]