3D Culture with Gelacell™ Nanofiber Scaffold Products

Gelacell™ 3D culture scaffolds provide unique material properties optimised to mimic natural ECM, promoting cell motility and attachment as well as nutrients transfer. Produced using the patented Halospin™ process, Gelacell™ 3D culture scaffolds are tuned to provide high porosity (>90% for inward cell migration), rigidity, flexibility and attachment surface. These scaffolds are fundamentally different from the less porous, planar and stiff fiber alignment characteristics observed with other scaffold production methods. Gelacell™ 3D culture scaffolds are very usefully positioned at the intersection between hard scaffold and soft hydrogel, ideal for research fields including in-vitro modelling, toxicity testing, tissue engineering, wound healing, drug release and organoid culture.

Gelacell™ biomaterial scaffolds are provided in convenient formats including sterile ready-to-use multiwell plates, mats and inserts. These products help you to achieve greater biological relevance by preserving natural cell shape, reducing cell stress and recreating the three dimensional cell:cell interactions more resemblant of native tissue. Gelacell™ nanofibrous scaffolds can also be used for the development of bioinstructive substrates for stem cell directed-differentiation protocols. Various pharmaceutical grade scaffold materials are proposed, including aligned fibers, each offering biocompatibility, biodegradability, non-toxicity, batch-to-batch consistency, and reproducibility.

Example scaffolds :

White paper with culture examples : here

Gallery of SEM images of Gelacell scaffolds here: SEMs

Long-term 3D culture plates (movable cell crown in each well)

Scaffolds fixed in a movable cell crown offer a convenient approach for maintaining a 3D culture for long periods. The cell crown inserts permit larger media volumes per well and enable fast and easy media changes when required. Can also be used for air-liquid interface culture, co-culture, complex tissue models such as lung and gut barrier, exosome secretion studies etc.

Gelacell 12 well plate with cell crowns

ORDERING INFORMATION : long-term culture plates with scaffolds in cell crowns

PLLA AlignedGC0805AL-CC06-BGC0805AL-CC12-BN/A
Gelatin GC0601RN-CC06-BGC0601RN-CC12-BEnquire
Gelatin AlignedEnquireEnquireEnquire

24-Well Plate with scaffold on PET discs

A 24-well plate containing pre-installed PLLA scaffolds each on a PET support disk. Suitable for medium term 3D culture. Gelacell 24-Well Plate video guidelines

ORDERING INFORMATION : 24-well plate with inserts

PLLA AlignedGC0805AL-WP24-A

Nanofiber scaffold inserts for your standard multiwell plates

Nanofibrous inserts for multiwell plates offer an easy, low cost way to test various materials with your cells growing in 3D. Cell seeding density per well between 10^4 and 10^7 cells. Suitable for medium term 3D culture.

ORDERING INFORMATION : scaffold inserts for well plates

PLLA ALIGNEDGC0805AL-IN06-AEnquireEnquire
GELATIN ALIGNEDEnquireEnquireEnquire

10 cm x 10 cm scaffold

10cm x 10cm Gelacell™ nanofibrous scaffold sheets are available in any of the materials indicated below, gamma sterilized. These economical scaffold mats may be cut and shaped for user-specific 3D culture and tissue engineering applications.

ORDER INFO: scaffold sheets

MaterialGelacell Sheet 10 cm x 10 cmThickness (µm)
PLLAGC0805RN-SC-C140 – 230
PLLA AlignedGC0805AL-SC-C120 – 220
PLGAGC0800RN-SC-C120 – 280
PLGA:PCLGC0801RN-SC-C120 – 200
PCLGC0701RN-SC-C250 – 400
GelatinGC0601RN-SC-C 550 – 750
Gelatin AlignedGC0601AL-SC-Cenquire

In-vitro derived 3D tissues grown in Gelacell scaffolds can be studied using standard techniques

  • Tissue processing fixation embedding and sectioning
  • Histological staining, in situ hybridisation
  • Bright-field microscopy and photographic imaging
  • Electron microscopy, DEM and TEM
  • Cryostat sectioning
  • Immunocytochemistry
  • Fluorescence microscopy, confocal, laser capture
  • Isolation of viable cells
  • Extraction of nucleic acid and total protein
  • Biochemical assays

Halospin™ nanofiber production technology

Halospinning is a new nanofiber solution-spinning method patented by Gelatex Technologies that is ideal for generating biocompatible scaffolds for 3D cell culture in a variety of materials.

Gelatex Technologies is a materials technology company specialized in the high-throughput manufacturing of nanofibrous materials using its proprietary halospinning technology – the world’s fastest and most cost-effective solution-spinning process.

Distributed by KDBIO s.a.s. in Europe.

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Easily measure glucose accurately in cell culture media

The GlucCell meter offers a convenient way to monitor glucose in media during long-term cell culture. Details available here.