Secretome collection from spheroids

Collect secretome from 20,000+ spheroids per plate

Microwell-in-macrowell technology enables the easy maintenance of thousands of spheroids on a single plate. For example, each well in the 6-well format plate can grow over 3000 uniform spheroids that share the same media. Conditioned media containing exosomes can readily be harvested from each of the 6 wells with one pipetting step per well. The SP5D plate is also available in 24-well plate format as shown below.

9000 spheroids

20184 spheroids

Product Page : SP5D 6-Well Plate

Simple procedure for all SP5D plate types – 6 well version shown below

PREPARE functional wells with 1 ml medium

ADD CELLS – 1 ml of single cell suspension

CULTIVATE spheroids in incubator

Product Page : SP5D 6-Well Plate

References:

Lisa-Marie Philipp, Pirsch Matthias, et al., Synentec Application Note : https://www.sp5d.com/cm/wp-content/uploads/SYNENTECkugelmeiers_sphericalplate_5d_FINAL.pdf

Nishant S.Kulkarni, Vivek Gupta, et al., Materials Science and Engineering
Journal, 2021 : https://doi.org/10.1016/j.msec.2021.111891

Laura Schreyer, Susanne Muehlich, et al., Cancers Journal, 2021 : https://doi.org/10.3390/cancers13215373

Tristen V. Tellman, Mary C. Farach-Carson, et al., International Journal of Molecular Sciences, 2021 : https://doi.org/10.3390/ijms22063218