Mass production of stem cell spheroids

Spheroids for tissue engineering

Spheroids provide promising approaches for tissue engineering. The greater multi-lineage differentiation capacity of adult stem cells cultured as spheroids continues to generate excitement and there has been an explosion in the number of related publications. Many methods have been described to generate spheroids with size and shape being key control parameters. The goal for tissue engineers is to reproducibly generate high numbers of uniform, size-tunable spheroids in a non-labour intensive way.

The rounded bottom, conical wells of Sphericalplate SP5D plates, are designed to generate genuinely spherical spheroids. The steep sides of the microwells promote rapid cellular aggregation.

Examples of stem cells successfully cultivated in SP5D plates

The SP5D 6-Well plate with 3364 microwells per well, as shown above, is an easily scalable method to consistently produce 2E4 to millions of stem cell spheroids. Here are examples of stem cells successfully cultivated in SP5D microwells :

  • Human bone marrow-derived mesenchymal stromal cells
  • Human adipose-derived mesenchymal stromal cells
  • Human amniotic epithelial cells

Product Page : SP5D 6-Well Plate

Simple procedure for all SP5D plate types – 6 well version shown below

PREPARE functional wells with 1 ml medium

ADD CELLS – 1 ml of single cell suspension

CULTIVATE spheroids in incubator

Product Page : SP5D 6-Well Plate

References:

Lisa-Marie Philipp, Pirsch Matthias, et al., Synentec Application Note : https://www.sp5d.com/cm/wp-content/uploads/SYNENTECkugelmeiers_sphericalplate_5d_FINAL.pdf

Nishant S.Kulkarni, Vivek Gupta, et al., Materials Science and Engineering
Journal, 2021 : https://doi.org/10.1016/j.msec.2021.111891

Laura Schreyer, Susanne Muehlich, et al., Cancers Journal, 2021 : https://doi.org/10.3390/cancers13215373

Tristen V. Tellman, Mary C. Farach-Carson, et al., International Journal of Molecular Sciences, 2021 : https://doi.org/10.3390/ijms22063218

References:

Eishi Aizawa, Anton Wutz, et al., Stem Cell Reports Journal, 2021. https://doi.org/10.1016/j.stemcr.2021.11.006

Siddharth Shanbhag, Salwa Suliman, et al., Frontiers Bioengineering
Biotechnology Journal, 2021. https://doi.org/10.3389/fbioe.2021.783468

Ingrid Zahn, Gundula Schulze-Tanzil, et al., Internation Journal of Molecular
Sciences, 2021. https://doi.org/10.3390/ijms222011011

Dominik Egger, Cornelia Kasper, et al., Engineering in Life Sciences Journal,
2021.https://doi.org/10.1002/elsc.202100097