G-Rex® Culture Plates

Once inoculated, non-adherent cells gravitate to the well base and settle above the G-Rex® gas exchange membrane forming a dense layer of cells after a few days. Cells remain well oxygenated and expand with little or no need for user intervention.

G-Rex® 6M-Well Plate

Easily expand 5 million non-adherent cells (such as T cells, NK cells, HSC) per well into up to 400 million cells per well in 7 to 10 days with no need for medium exchange.

Primary application :

  • Cell and gene-modified cell research and manufacturing process development
  • Allows activation, transduction, and expansion
  • Process development protocols directly scale up to clinical doses in larger G-Rex® bioreactors

Gas permeable membrane surface area per well  10 cm2

Media volume per well  100 ml

Cat.# 80660M

Product page G-Rex® 6M-Well Plate

G-Rex® 6-Well Plate

Expand 5 million non-adherent cells per well to between 200 and 400 million cells per well in about 10 days with only one exchange of medium.

Example application : optimize a protocol for the production of monoclonal antibodies by hybridoma technology, then scale-up with the 1 Litre EZ-Flask.

Gas permeable membrane surface area per well  10cm2

Media volume per well  35 ml

Cat.# KDW0026

G-Rex 6 well plate

G-Rex® 24-Well Plate

Expand 1 million non-adherent cells per well into between 40 and 80 million cells per well in about 10 days with only one exchange of medium.

Gas permeable membrane surface area per well 2 cm2 

Media volume per well 7ml 

Cat.# KDW0024

G-Rex 24

Gas permeable base membrane

A gas-permeable silicone membrane at the base of each well ensures cells remain well oxygenated and expand continuously. This means minimal user intervention is required without the need for frequent media change. For example : inoculate G-Rex® 6 with 5e6 cells and achieve over 100X cell expansion in 7 to 10 days.

Production of monoclonal antibodies by hybridoma technology

No passaging required

G-Rex® culture plates enable the expansion of non-adherent cells to very high numbers without the need for passaging. Scalable method to larger volumes, please enquire.

T-Cells, NK Cells, HSC

G-Rex® 6M (100ml well volume) is used for expansion of T-Cells, NK-Cells etc with no need for a medium change. G-Rex® 6M plates are used in cell and gene-modified cell research and manufacturing process development. The device allows for activation, transduction, and expansion. The protocols developed with G-Rex® 6M directly scale up to clinical doses in larger G-Rex® bioreactors.

Hybridoma development

Antibody developers use G-Rex® 6 and 24 well plates as a convenient way to make small amounts of mAb and compare hybridoma clones.

Continuous culture of organoids

We were excited to see the 2021 paper “Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids” in which the authors cultured multi-tissue hiPSC-derived organoids for many weeks continuously in a G-Rex bioreactor  doi: https://doi.org/10.1101/2021.09.21.461213

Cat.#DescriptionVolume ml per wellMembrane area cm2 per well
80660MG-Rex 6M Well Plate10010
KDW0026G-Rex 63510
KDW0024G-Rex 2472

CAR-T for solid tumour research – use of spheroids

CAR-T (Chimeric Antigen Receptor T-cell) therapy has shown remarkable success in treating certain hematologic malignancies, but its application to solid tumors faces several significant challenges. Incorporating 3D culture systems, such as spheroids, into CAR-T therapy research for solid tumors provides a versatile platform to better understand and overcome these challenges. By closely mimicking the tumor’s biological environment, these 3D tissue models offer critical insights that can ultimately lead to enhancing the efficacy, safety, and scalability of CAR-T therapies.

Sphericalplate 5D for tumoroid testing

SP5D spheroid plate – 25 technical replicates per well

Precision Oncology Research News

SphericalPlate 5D - 25 tumoroids/spheroids per well The Advantage of SP5D Patient-Derived Tumor Organoids and Multicellular Tumor Spheroids Patient derived tumoroids and tumour spheroids provide a revolutionary tool that opens the way to [...]