Once inoculated, suspension cells such as hybridomas will gravitate to the well base and settle above the G-Rex gas exchange membrane forming a dense layer of cells after a few days. Cells remain well oxygenated and expand without user intervention, apart from occasional removal of media and re-feeding. A single well of G-Rex 6-Well Plate can be equivalent to 2 or 3 x T175 flasks when generating monoclonal antibody.

Gas permeable base membrane

A gas-permeable silicone membrane at the base of each well ensures cells remain well oxygenated and expand continuously. This means minimal user intervention is required without the need for frequent media change. For example : inoculate G-Rex 6 with 5e6 cells and achieve over 100X expansion. The EZ-Flask (1 Litre) represents 20 x scale-up of a single well of the G-Rex 6 plate.

No passaging required

G-Rex culture plates enable the expansion of hybridomas and other non-adherent cells to very high numbers without the need for passaging. For example, each 35ml well of a G-Rex 6 Plate acts as a mini-bioreactor yielding 3 – 6 x more monoclonal antibody than a T-175 flask when growing a hybridoma. See example data below

Ideal for comparing hybridoma clones

Inoculate G-Rex 6 with 1.25 million cells / well …and 10 days later, with just one media exchange, each well can yield 200 to 400 million cells.

Continuous culture of organoids

We were excited to see the 2021 paper “Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids” in which the authors cultured multi-tissue organoids for many weeks continuously in a G-Rex bioreactor  doi: https://doi.org/10.1101/2021.09.21.461213