G-Rex Hybridoma Culture Plates

Generate useful amounts of monoclonal antibody from each well

No passaging required

G-Rex culture plates enable the expansion of hybridomas to very high numbers without the need for passaging.

Each 35ml well of a G-Rex 6 Plate acts as a mini-bioreactor yielding 3 – 6 x more monoclonal antibody than a T-175 flask. See example data below.

Gas permeable base membrane

A gas-permeable silicone membrane at the base of each well ensures cells are oxygenated despite the deep well design.

The only limitation on cell proliferation is the volume and type of media. Exponential growth takes place over a period of days with minimal user intervention.

Production of monoclonal antibodies by hybridoma technology

Ideal for comparing hybridoma clones

Inoculate G-Rex 6 with 1.25 million cells / well …and 10 days later, with just one media exchange, each well can yield 200 to 400 million cells.

“We tested your G-Rex 6 well Plates with very good results. We used newly etablished monoclonal cell lines to generate the first amounts of purified antibodies”

mAb production lab in Germany

We tried two rat mAbs, #IBL-11 (against an undefined mouse lymphoid stromal marker) and #IBL-12 against mouse MARCO antigen, both developed in our lab. Considering the speed for achieving several mgs of each, I am very satisfied with the performance of G-rex, and the ease of operation.

We could clearly collect and re-initiate the growth by removing weekly (a) 30 ml of supernatant, adding fresh, and (b) three days later collecting 32 ml (out of 35 total) of another spent medium with cells (so we had twice-a-week SN harvest) for three weeks.

Attached, the actual yields obtained.

Péter Balogh, MD, PhD, University of Pécs

I have just performed our experiments and I have to inform you that I’ve obtained very good amounts of antibodies from the G-Rex6. Since, I got a much larger amount from this than from my normal protocol I couldn’t quantify it yet and I will have to dilute it next time. For now we would like to get 20 EZ flasks and 6 G-Rex6 well plates since we are starting a new project for antibody production. We would like to implement these production systems right away.

Antibody Porduction Lab - Germany

G-Rex® 6 Plate

Expand 5 million cells per well into between 200 and 400 million cells per well in about 10 days with only one exchange of medium.

Test out different protocols for monoclonal antibody development.

10cm2 gas permeable membrane surface area per well

35 ml fill volume per well

Cat.# KDW0026

G-Rex 6 well plate

G-Rex® 24 Plate

Expand 1 million cells per well into between 40 and 80 million cells per well in about 10 days with only one exchange of medium.

2 cm2 gas permeable membrane surface area per well

Test out different protocols for the production of monoclonal antibodies by hybridoma technology

7ml fill volume per well

Cat.# KDW0024

G-Rex 24

Grow >1e9 cells without passaging

EZ Flask bioreactor is a 1 Litre single compartment flask that enables 100 million suspension cells to be expanded to over 4 billion cells in about 10 days with only one exchange of medium, e.g. hybridomas, CHO, Sf9, Jurkat…

200 cm2 gas permeable membrane surface

1 Litre capacity

Cat.# KDW0010

Ez-Flask for in vitro monoclonal antibody production

Compare different methods for producing monoclonal antibodies by hybridoma technology.

Cat.#DescriptionVolume mlMembrane area cm2
KDW0026G-Rex 635/well10/well
KDW0024G-Rex 247/well2/well
KDW0010EZ Flask1000200
EZ Flask is manufactured by Wilson Wolf (USA)
Distributed by KDBIO in Europe (any application apart from T-Cell Therapy applications) including UK, NO and CH

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