G-Rex® Culture Plates
G-Rex® culture plates offer large well volumes with a base gas exchange membrane and are therefore ideal for easily expanding non-adherent cells such as T-Cells, NK-Cells or hybridomas. Direct scale-up to larger bioreactor systems is possible with this technology. How does it work? After inoculation, cells gravitate to the well base and settle above the G-Rex® gas exchange membrane. Basal gas exchange technology circumvents the usual restriction on height of media meaning that cells can grow uninterrupted for several days in a large volume of media. A dense layer of cells is obtained at the base of each well which makes it easy to harvest cells in a small final volume.
G-Rex® 6-Well Plate
Expand 5 million non-adherent cells per well to between 200 and 400 million cells per well in about 10 days with only one exchange of medium.
Gas permeable membrane surface area per well 10cm2
Media volume per well 35 ml
Cat.# KDW0026 (80240M)
G-Rex® 24-Well Plate
Expand 1 million non-adherent cells per well into between 40 and 80 million cells per well in about 10 days with only one exchange of medium.
Gas permeable membrane surface area per well 2 cm2
Media volume per well 7ml
Cat.# KDW0024 (80192M)
G-Rex® 6M-Well Plate
Easily expand 5 million non-adherent cells (such as T cells, NK cells, HSC) per well into up to 400 million cells per well in 7 to 10 days with no need for medium exchange.
Primary application :
- Cell and gene-modified cell research and manufacturing process development
- Allows activation, transduction, and expansion
- Process development protocols directly scale up to clinical doses in larger G-Rex® bioreactors
Gas permeable membrane surface area per well 10 cm2
Media volume per well 100 ml
Cat.# 80660M
Product page G-Rex® 6M-Well Plate
Gas permeable base membrane
A gas-permeable silicone membrane at the base of each well ensures cells remain well oxygenated and expand continuously. This means minimal user intervention is required without the need for frequent media change (explanatory PDF). For example : inoculate G-Rex® 6 with 5e6 cells and achieve over 100X cell expansion in 7 to 10 days with only one media change.
No passaging required
G-Rex® culture plates enable the expansion of non-adherent cells to very high numbers without the need for passaging. Scalable method to larger volumes, please enquire.
T-Cells, NK Cells, HSC
G-Rex® 6M (100ml well volume) is used for expansion of T-Cells, NK-Cells etc with no need for a medium change. G-Rex® 6M plates are used in cell and gene-modified cell research and manufacturing process development. The device allows for activation, transduction, and expansion. The protocols developed with G-Rex® 6M directly scale up to clinical doses in larger G-Rex® bioreactors.
Hybridoma development
Antibody developers use G-Rex® 6 and 24 well plates as a convenient way to make small amounts of mAb and compare hybridoma clones.
Continuous culture of organoids
Example : “Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids”. The authors cultured multi-tissue hiPSC-derived organoids for many weeks continuously in a G-Rex bioreactor doi: https://doi.org/10.1101/2021.09.21.461213
| Cat.# | Description | Volume ml per well | Membrane area cm2 per well |
|---|---|---|---|
| 80660M | G-Rex 6M Well Plate | 100 | 10 |
| KDW0026 | G-Rex 6 | 35 | 10 |
| KDW0024 | G-Rex 24 | 7 | 2 |
CAR-T for solid tumour research – use of spheroids
CAR-T (Chimeric Antigen Receptor T-cell) therapy has shown remarkable success in treating certain hematologic malignancies, but its application to solid tumors faces several significant challenges. Incorporating 3D culture systems, such as spheroids, into CAR-T therapy research for solid tumors provides a versatile platform to better understand and overcome these challenges. By closely mimicking the tumor’s biological environment, these 3D tissue models offer critical insights that can ultimately lead to enhancing the efficacy, safety, and scalability of CAR-T therapies.
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