G-Rex® Culture Plates
Expansion of non-adherent cells to high numbers with no need to change media
G-Rex® culture plates are used to obtain 40X – 80X expansion of non-adherent cells without changing media which is an ideal way for growing high numbers of T-Cells, NK-Cells or hybridomas. Direct scale-up to larger bioreactor systems using G-Rex® basal gas exchange membrane technology is straightforward. Please enquire.
How does it work?
After inoculation, cells will gravitate downwards and settle above the G-Rex® gas exchange membrane. This innovative culture oxygenation system circumvents the usual restriction on media height, meaning that cells can multiply uninterrupted for several days in a large volume of media. A dense layer of cells is obtained at the base of each well which makes it easy to harvest cells in a small final volume. The G-Rex 24-w plate well volume is 7ml/well, the G-Rex 6-w plate well volume is 35ml/well and each well of the 6M version has 100ml volume.
G-Rex® 6-Well Plate
Expand 5 million non-adherent cells per well to between 200 and 400 million cells per well in about 10 days with only one exchange of medium.
Gas permeable membrane surface area per well 10cm2
Media volume per well 35 ml
Cat.# KDW0026 (80240M)
G-Rex® 24-Well Plate
Expand 1 million non-adherent cells per well into between 40 and 80 million cells per well in about 10 days with only one exchange of medium.
Gas permeable membrane surface area per well 2 cm2
Media volume per well 8ml.
Cat.# KDW0024 (80192M)
G-Rex® 6M-Well Plate
Easily expand 5 million non-adherent cells (such as T cells, NK cells, HSC) per well into up to 400 million cells per well in 7 to 10 days with no need for medium exchange.
Primary application :
- Cell and gene-modified cell research and manufacturing process development
- Allows activation, transduction, and expansion
- Process development protocols directly scale up to clinical doses in larger G-Rex® bioreactors
Gas permeable membrane surface area per well 10 cm2
Media volume per well 100 ml
Cat.# 80660M
Product page G-Rex® 6M-Well Plate
G-Rex®100M Open System R.U.O., Cat.# RU81100
100cm² gas permeable membrane surface with 1000 mL media capacity. Expand 50 million cells into between 2 to 4 billion cells in about 10 days with NO medium exchange. [GAMMA IRRADIATED. For non-clinical, non-therapeutic, research use only.]
KDBIO supplies the G-Rex® range to EU laboratories.
Our quotes in Euros cover all tariffs and costs associated with international shipping of products manufactured by WilsonWolf Manufacturing LLC (USA)
No passaging required
G-Rex® culture plates enable the expansion of non-adherent cells to very high numbers without the need for passaging. Scalable method to larger volumes, see WilsonWolf Manufacturing product listing.
T-Cells, NK Cells, HSC
G-Rex® 6M (100ml well volume) is used for expansion of T-Cells, NK-Cells etc with no need for a medium change. G-Rex® 6M plates are used in cell and gene-modified cell research and manufacturing process development. The device allows for activation, transduction, and expansion. The protocols developed with G-Rex® 6M directly scale up to clinical doses in larger G-Rex® bioreactors.
Hybridoma development
Antibody developers use G-Rex® 6 and 24 well plates as a convenient way to make small amounts of mAb and compare hybridoma clones.
Gas permeable base membrane
A gas-permeable base silicone membrane ensures cells remain well oxygenated and expand continuously. This means minimal user intervention is required without the need for frequent media change (explanatory PDF). For example : inoculate G-Rex® 6 with 5e6 cells and achieve over 100X cell expansion in 7 to 10 days with only one media change.
