G-Rex Cell Culture Plates
Generate useful amounts of monoclonal antibody from several hybridoma clones cultured simultaneously
1) No passaging required
The G-Rex cell culture plates enable the expansion of non-adherent cells to very high numbers without the need for passaging. The production of monoclonal antibodies by hybridoma technology is perfectly suited within the G-Rex plates.
For example, when comparing candidate hybridoma clones, a 35ml well of a G-Rex 6 Plate, without optimisation, might yield 3 – 6 x more monoclonal antibody than a T-175 flask.
2) Gas permeable base membrane
A gas-permeable silicone membrane at the base of each well ensures cells are oxygenated despite the deep well design.
The only limitation on cell proliferation is the volume and type of media. Each well in a G-Rex cell culture plate behaves like a “mini bioreactor”.
Each well base has a surface area of 10cm2. This allows for increased oxygenation and proliferation and thus a higher production of monoclonal antibodies.
3) High cell numbers in just a few days
Exponential growth takes place over a period of days with minimal user intervention. Inoculate G-Rex 6 with 1.25 million cells / well …and 10 days later, with just one media exchange, each well can yield 200 to 400 million cells.
“We tested your G-Rex 6 well Plates with very good results. We used newly etablished monoclonal cell lines to generate the first amounts of purified antibodies”
We tried two rat mAbs, #IBL-11 (against an undefined mouse lymphoid stromal marker) and #IBL-12 against mouse MARCO antigen, both developed in our lab. Considering the speed for achieving several mgs of each, I am very satisfied with the performance of G-rex, and the ease of operation.
We could clearly collect and re-initiate the growth by removing weekly (a) 30 ml of supernatant, adding fresh, and (b) three days later collecting 32 ml (out of 35 total) of another spent medium with cells (so we had twice-a-week SN harvest) for three weeks.
I have just performed our experiments and I have to inform you that I’ve obtained very good amounts of antibodies from the G-Rex6. Since, I got a much larger amount from this than from my normal protocol I couldn’t quantify it yet and I will have to dilute it next time. For now we would like to get 20 EZ flasks and 6 G-Rex6 well plates since we are starting a new project for antibody production. We would like to implement these production systems right away.
G-Rex® 6 Plate
Expand 5 million cells per well into between 200 and 400 million cells per well in about 10 days with only one exchange of medium.
Test out different protocols for monoclonal antibody development.
10cm2 gas permeable membrane surface area per well
35 ml fill volume per well
Cat.# KDW0026
G-Rex® 24 Plate
Expand 1 million cells per well into between 40 and 80 million cells per well in about 10 days with only one exchange of medium.
2 cm2 gas permeable membrane surface area per well
Test out different protocols for the production of monoclonal antibodies by hybridoma technology
7ml fill volume per well
Cat.# KDW0024
Grow >1e9 cells without passaging
EZ Flask bioreactor is a 1 Litre single compartment flask that enables 100 million suspension cells to be expanded to over 4 billion cells in about 10 days with only one exchange of medium, e.g. hybridomas, CHO, Sf9, Jurkat…
200 cm2 gas permeable membrane surface
1 Litre capacity
Cat.# KDW0010
Compare different methods for producing monoclonal antibodies by hybridoma technology.
| Cat.# | Description | Volume ml | Membrane area cm2 |
|---|---|---|---|
| KDW0026 | G-Rex 6 | 35/well | 10/well |
| KDW0024 | G-Rex 24 | 7/well | 2/well |
| KDW0010 | EZ Flask | 1000 | 200 |
EZ Flask is manufactured by Wilson Wolf (USA)
Distributed by KDBIO in Europe (any application apart from T-Cell Therapy applications) including UK, NO and CH
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