Sphericalplate 5D® 96well – 25 spheroids per well

Tumor spheroids – Tumoroids – Stem cell clustering

25 spheroid replicates per well

The Sphericalplate 5D® 96w spheroid plate generates 25 spheroids per well, in microwells, providing 200 spheroids in each removable 8-well strip. This plate design gives a convenience when working with small amounts of patient-derived material and a remarkable improvement in the statistical validity of dose-response data. Thanks to the unique round-bottom microwells, cells cluster reliably into consistently sized spheroids. The plate allows for high quality imaging and easy media changes. It’s also simple to recover 25 spheroids from each well for further processing.

Cat.#  KDK096

Easy media change for multiple spheroids per well

25 spheroids per well in round-bottom microwells (non-adhesive). Spheroid diameters up to 500µm.

Image showing how to generate more spheroid replicates on a standard size spheroid plate

Long term spheroid culture

“The SP5D 96W plate enabled us to grow primary human hepatocytes for 21 days, with 25 replicates per well.» [PDF]

Sabine Gerbal-Chaloine, INSERM

ADVANTAGES  :

  • 25 spheroid replicates / well
  • easy media changes
  • 12 strips of 8 wells = 200 spheroids per strip
  • strips are removable and can be used individually
  • round bottom microwells for uniformity
  • anti-adhesive
  • easy imaging, high optical quality plastic
  • easy to collect spheroids from each well

EUROPEAN DISTRIBUTOR

Do you need even more spheroids per well ?

With the Sphericalplate 5D plates shown below you can easily grow

9000 or 20’184 spheroids per plate [..MORE INFO)

Specifications Cat.#  KDK096

Strip material: Transparent Cyclic Olefin Copolymer (COC)

Format : Strip for 96 well frame (COC)

Outer dimensions of frame:  127.8 x 85.6 x 14.3 mm (standard SBS footprint)

Number of microwells per well:  25

Number of microwells per strip: 200

Maximum volume per well: 0.4 ml

Working volume per well: 0.1 – 0.2 ml

Surface modification: Non Fouling / Low adhesion coating

Lid and Frame material: Polystyrene (PS)

Sterility: X-Ray irradiated

Technical questions : [email protected]

Manufacturer : Kugelmeiers Ltd. (Switzerland), distributed in EU by KDBIO S.A.S.