Sphericalplate 5D® 96well plate for 2400 spheroids

Greater statistical power with 25 spheroids PER WELL

25 spheroids per well

The Sphericalplate 5D® 96w spheroid plate generates 25 spheroids per well, in microwells, providing 200 spheroids in each removable 8-well strip. This design increases the number of spheroids with no extra effort required, providing an easy way to improve statistical power. The removable strips make this plate ideal for working with small amounts of patient-derived material. In addition, thanks to the unique round-bottom microwells, cells cluster reliably into consistently sized spheroids. Produced with optically clear COC plastic to facilitate microscopic analysis. Research-use only : ideal for functional precision oncology research.

Cat.# KDK096

ADVANTAGES :

Easily generate 25 spheroids per well
Obtain a more precise estimation of treatment effects compared to standard plates
12 removable strips of 8 wells
Strips are removable and can be used individually
Round bottom microwells for uniformity
Anti-adhesive
Easy imaging, high optical quality plastic
Easy to collect 25 spheroids from each well for IHC

Comparison Table : Kugelmeiers SP5D vs. 96-Well ULA plates

Feature	Traditional ULA	Kugelmeiers SP5D 96-Well
Spheroids per well	1	25
Spheroids per plate	96	up to 2,400
Formation mechanism	Passive aggregation on non-adhesive coating	Passive aggregation in patterned microcavities with proprietary nanocoating that prevents cell adhesion
Pretreatment needed	None	None
Uniformity	Depends on cell type	Depends on cell type. Designed for low size variance across replicates WITHIN a well.
Size	Large diameter spheroids possible	Up to max 500µm diameter
Replicate structure per condition tested	Technical replication requires more wells	25 nested technical replicates per well, formed under matched microenvironmental conditions
Media exchange	Straightforward pipetting	Straightforward pipetting; microwells retain clusters during exchange
Format	96-well block, fixed	12 removable 8-well strips – allows partial plate use
Downstream retrieval	Single spheroid per well	Easy retrieval of 25 spheroids per well
Imaging	Depends on plate	Reported imaging compatibility with Incucyte

“The SP5D 96W plate enabled us to grow primary human hepatocytes for 21 days, with 25 replicates per well.» [PDF]

Sabine Gerbal-Chaloine, INSERM

“Indeed, we were able to test your plate in our Incucyte and our experiments were a success!…with more statistical power than in standard 96-well plates”

Laboratory in France, INSERM