It has been shown (1) that iPSC derived multi-tissue organoids can be grown for up to 30 weeks in G-Rex bioreactors which provide a continuous exchange of gas at the cell/tissue interface via a basal gas-exchange membrane. Little routine maintenance is required apart from refreshing media every few days. G-Rex culture systems are produced [...]

Check out this new range of hollow fiber ultrafilters from KDBIO. Attractive prices. Various sizes of hollow fiber cartridge, available sterile or non-sterile, for concentration/diafiltration of cytokines and larger proteins/viruses, and for cell perfusion experiments. Datasheets via links on product page. Product Page

Prepare 1 Litre of monoclonal antibody from hybridoma using EZ-Flask with serum free media Add 250ml serum free hybridoma media to EZ-Flask Inoculate with 10^7 hybridomas Wait 1 week without disturbing EZ-Flask, then add 250ml media Wait 1 week without disturbing EZ-Flask, then add 250ml media Wait 1 week without disturbing EZ-Flask, then add [...]

Culturing hybridoma cell lines for monoclonal antibody production in EZ-Flask saves user time, incubator space and provides easy and economical protein expression. Below you will find detailed descriptions of the major features of this simple yet powerful bioreactor. Gas exchange technology The issue with traditional T-Flask cell culture is the limited amount of media, [...]

In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines. Following the successful fusion, clone selection and weaning from media supplements, candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well). The [...]