Transient or stable expression of monoclonal antibody?

…your decision may depend on cell culture technology The transient expression of monoclonal antibodies is an essential step for early characterization studies but to produce 500 – 1000 mg of mAb requires a huge quantity of plasmid and transfection agent. The inherent heterogeneity of the cells means there’s no guarantee that post-translational modifications will [...]

By |2022-11-04T13:38:02+00:00November 4th, 2022|blog|0 Comments

How to produce antibodies, cytokines, complex proteins or exosomes for translational research projects

Translational research projects are sometimes hampered by low productivity of an essential cell-secreted agent; this might be, for example, a monoclonal antibody, a cytokine or a more complex recombinant protein or perhaps even exosomes that need to be harvested from MSCs or another specific cell type. The incubator space dedicated to culture flasks for [...]

By |2022-11-20T10:33:44+00:00October 21st, 2022|blog|0 Comments

Amniotic Fluid Stem Cells Extracellular Vesicles – Scalability of Production and Bio-Activity – 3D vs 2D culture

Poster Abstract EV clinical translation is constrained by limitations in scale-up of EVs production. Hollow fiber bioreactors (HFBR) support the culture of large numbers of cells, at high densities, producing significant numbers of EVs at high concentration. The high cell densities present in a HFBR can facilitate the use of xeno-free/chemically defined media, such as [...]

By |2022-08-23T10:32:17+00:00August 23rd, 2022|blog|0 Comments

BioBUZZ on FiberCell

BioBUZZ Article about hollow fibre culture of cells "If you’ve worked in cell culture, you’ve surely had experience dealing with tissue culture flasks. These plastic vessels are the staple of cell culture work, but they definitely have their share of shortcomings. Having to transfer your cells to gradually larger flasks to scale up. Carefully [...]

By |2022-04-26T12:47:39+00:00April 26th, 2022|blog|0 Comments

FiberCell Hollow Fibre Bioreactor in Freshney, the Bible of Cell Culture

Freshney's 8th Edition of "Culture of Animal Cells" contains an entire section about the culture of mammalian cells in a FiberCell Hollow Fibre Bioreactor. Considered by many prominent scientists to be the Bible of cell culture it is not surprising that Freshney's 8th edition contains a dedicated detailed section about Hollow Fibre technology. [...]

By |2022-03-23T12:37:46+00:00March 16th, 2022|blog|0 Comments

Monoclonal antibody scale-up guide

Two methods for scaling up mAb production in your own lab Would you like better antibody yields? Are you lacking incubator space for T-Flasks? Tired of passaging cells? Need a better solution than roller bottles? Check out this scale-up guide.

By |2022-03-23T12:37:46+00:00February 28th, 2022|blog|0 Comments

piggyBac transposon-based expression system in combination with FiberCell hollow fibre bioreactor enables remarkable levels of production of HexM expression

100 x increase in protein yield accelerates ERT preclinical research Preclinical studies with potential therapeutic recombinant proteins can be hampered by difficulties with expression and scale-up in the laboratory. A group at the Department of Manitoba, Canada, used the piggyBac expression system in conjunction with the compact FiberCell hollow fibre bioreactor to generate >500mg [...]

By |2022-03-23T12:37:47+00:00December 26th, 2021|blog|0 Comments

Improving the Drug Development Pipeline for Mycobacteria

[Review]   Modelling Antibiotic Exposure in the Hollow Fibre Infection Model Maitra, A.; Solanki, P.;Sadouki, Z.; McHugh, T.D.;Kloprogge, F. Improving the Drug Development Pipeline for Mycobacteria: Modelling Antibiotic Exposure in the Hollow Fibre Infection Model. Antibiotics 2021, 10, 1515. https://doi.org/10.3390/antibiotics10121515 Mycobacterial infections are difficult to treat, requiring a combination of drugs and lengthy treatment times, [...]

By |2022-03-23T12:37:47+00:00December 20th, 2021|blog, HFIM News|0 Comments
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