BioBUZZ on FiberCell

BioBUZZ Article about hollow fibre culture of cells "If you’ve worked in cell culture, you’ve surely had experience dealing with tissue culture flasks. These plastic vessels are the staple of cell culture work, but they definitely have their share of shortcomings. Having to transfer your cells to gradually larger flasks to scale up. Carefully [...]

By |2022-04-26T12:47:39+00:00April 26th, 2022|blog|0 Comments

FiberCell Hollow Fibre Bioreactor in Freshney, the Bible of Cell Culture

Freshney's 8th Edition of "Culture of Animal Cells" contains an entire section about the culture of mammalian cells in a FiberCell Hollow Fibre Bioreactor. Considered by many prominent scientists to be the Bible of cell culture it is not surprising that Freshney's 8th edition contains a dedicated detailed section about Hollow Fibre technology. [...]

By |2022-03-23T12:37:46+00:00March 16th, 2022|blog|0 Comments

Monoclonal antibody scale-up guide

Two methods for scaling up mAb production in your own lab Would you like better antibody yields? Are you lacking incubator space for T-Flasks? Tired of passaging cells? Need a better solution than roller bottles? Check out this scale-up guide.

By |2022-03-23T12:37:46+00:00February 28th, 2022|blog|0 Comments

piggyBac transposon-based expression system in combination with FiberCell hollow fibre bioreactor enables remarkable levels of production of HexM expression

100 x increase in protein yield accelerates ERT preclinical research Preclinical studies with potential therapeutic recombinant proteins can be hampered by difficulties with expression and scale-up in the laboratory. A group at the Department of Manitoba, Canada, used the piggyBac expression system in conjunction with the compact FiberCell hollow fibre bioreactor to generate >500mg [...]

By |2022-03-23T12:37:47+00:00December 26th, 2021|blog|0 Comments

Improving the Drug Development Pipeline for Mycobacteria

[Review]   Modelling Antibiotic Exposure in the Hollow Fibre Infection Model Maitra, A.; Solanki, P.;Sadouki, Z.; McHugh, T.D.;Kloprogge, F. Improving the Drug Development Pipeline for Mycobacteria: Modelling Antibiotic Exposure in the Hollow Fibre Infection Model. Antibiotics 2021, 10, 1515. https://doi.org/10.3390/antibiotics10121515 Mycobacterial infections are difficult to treat, requiring a combination of drugs and lengthy treatment times, [...]

By |2022-03-23T12:37:47+00:00December 20th, 2021|blog, HFIM News|0 Comments

Protocol for continuous culture of mesenchymal stem cells at high density in a hollow fibre bioreactor to produce concentrated exosomes

For laboratories wishing to produce large amounts of extracellular vesicles (exosomes) from MSCs using a hollow fibre bioreactor, FiberCell Systems has now published a preliminary protocol based on feedback from users in Europe and USA. If you need to quickly generate concentrated and pure EVs from MSCs this document is definitely a "must read". [...]

By |2022-03-23T12:37:47+00:00November 2nd, 2021|blog|0 Comments

Media advice for celline flask production of monoclonal antibodies

High density celline flask culture of hybridomas is made more efficient by completely eliminating serum from both compartments. A serum replacement called CDM-HD or "Chemically-Defined Media for High Density Culture" is specifically designed for high density cultures and is supplied in Europe exclusively through KDBIO. It's important to note that this simplified media only [...]

By |2022-03-23T12:37:48+00:00October 3rd, 2021|blog|0 Comments

EZ-Flask: Culturing hybridoma cell lines for monoclonal antibody production

Culturing hybridoma cell lines for monoclonal antibody production in EZ-Flask saves user time, incubator space and provides easy and economical protein expression. Below you will find detailed descriptions of the major features of this simple yet powerful bioreactor. Gas exchange technology The issue with traditional T-Flask cell culture is the limited amount of media, [...]

By |2022-03-23T12:37:48+00:00August 30th, 2021|blog|0 Comments

Development of monoclonal antibody using G-Rex Plates

In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines. Following the successful fusion, clone selection and weaning from media supplements, candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well). The cell [...]

By |2022-06-15T13:50:34+00:00August 19th, 2021|blog|0 Comments
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