Culturing hybridoma cell lines for monoclonal antibody production in EZ-Flask saves user time, incubator space and provides easy and economical protein expression. Below you will find detailed descriptions of the major features of this simple yet powerful bioreactor. Gas exchange technology The issue with traditional T-Flask cell culture is the limited amount of media, [...]
In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines. Following the successful fusion, clone selection and weaning from media supplements, candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well). The cell [...]
Monoclonal antibody production (hybridoma) in different systems Monoclonal antibody production can be achieved using various culture systems. Depending on the yield you are looking for you might need a different type of system. KDBIO offers 3 scale-up cell culture systems from small batch quantities up to continuous gram production quantities. Below you will find [...]
ISEV2021 provides a great opportunity to discuss 3D CM-EVs production Large amounts of EVs can be obtained from practically any type of cell, including mesenchymal stems cells, maintained over a few weeks at high density in a FiberCell Hollow Fibre Bioreactor. A medium size 20ml hollow fibre cartridge (C2011) offers 4000cm2 of porous fibre surface [...]
New YouTube Channel - Hollow fibre bioreactor technology and applications KDBIO has launched a Youtube channel with short videos to explain hollow fibre bioreactor technology and the advantages this offers for various applications. KDBIO YouTube Channel
Access lists of relevant references / Extracellular vesicles production / Monoclonal antibodies production / Recombinant proteins production / Secretome from 3D cultures / Virus production
CDM-HD : chemically-defined media for high density culture Once cells are growing at high density in a FiberCell bioreactor it is generally very easy to switch over to CDM-HD (DMEM/10%CMD-HD) which is ideal for generating cell-secreted products such as monoclonal antibodies or extracellular vesicles.