Freshney's 8th Edition of "Culture of Animal Cells" contains an entire section about the culture of mammalian cells in a FiberCell Hollow Fibre Bioreactor. Considered by many prominent scientists to be the Bible of cell culture it is not surprising that Freshney's 8th edition contains a dedicated detailed section about Hollow Fibre technology. [...]
Two methods for scaling up mAb production in your own lab Would you like better antibody yields? Are you lacking incubator space for T-Flasks? Tired of passaging cells? Need a better solution than roller bottles? Check out this scale-up guide.
100 x increase in protein yield accelerates ERT preclinical research Preclinical studies with potential therapeutic recombinant proteins can be hampered by difficulties with expression and scale-up in the laboratory. A group at the Department of Manitoba, Canada, used the piggyBac expression system in conjunction with the compact FiberCell hollow fibre bioreactor to generate >500mg [...]
[Review] Modelling Antibiotic Exposure in the Hollow Fibre Infection Model Maitra, A.; Solanki, P.;Sadouki, Z.; McHugh, T.D.;Kloprogge, F. Improving the Drug Development Pipeline for Mycobacteria: Modelling Antibiotic Exposure in the Hollow Fibre Infection Model. Antibiotics 2021, 10, 1515. https://doi.org/10.3390/antibiotics10121515 Mycobacterial infections are difficult to treat, requiring a combination of drugs and lengthy treatment times, [...]
For laboratories wishing to produce large amounts of extracellular vesicles (exosomes) from MSCs using a hollow fibre bioreactor, FiberCell Systems has now published a preliminary protocol based on feedback from users in Europe and USA. If you need to quickly generate concentrated and pure EVs from MSCs this document is definitely a "must read". [...]
Standard therapy of Mycobacterium avium complex pulmonary disease shows limited efficacy in an open source hollow fibre system that simulates human plasma and epithelial lining fluid pharmacokinetics : Ruth MM. et al.; Clinical Microbiology and Infection 2021 [abstract] Note : Supplementary information provided with this publication about the practical aspects of the set-up is helpful [...]
High density celline flask culture of hybridomas is made more efficient by completely eliminating serum from both compartments. A serum replacement called CDM-HD or "Chemically-Defined Media for High Density Culture" is specifically designed for high density cultures and is supplied in Europe exclusively through KDBIO. It's important to note that this simplified media only [...]
Le concept La production d'anticorps monoclonaux grâce à EZ-Flask (Flacon "Easy" en Américain) est grandement facilitée par le fait qu'il n'y est aucun repiquage nécessaire. Une grande concentration est possible dans son volume de 1 litre. Ses avantages majeurs sont l'augmentation significative de la production d'anticorps monoclonaux comparé à d'autres flacons de culture disponibles [...]
KDBIO Virtual Exhibition Stand at ECI2021 IN YOUR OWN LAB - produce significant amounts of monoclonal antibodies, recombinant proteins or extracellular vesicles using a FiberCell hollow fibre bioreactor. See also EZ-Flask for growing high numbers of hybridomas without passaging.
Culturing hybridoma cell lines for monoclonal antibody production in EZ-Flask saves user time, incubator space and provides easy and economical protein expression. Below you will find detailed descriptions of the major features of this simple yet powerful bioreactor. Gas exchange technology The issue with traditional T-Flask cell culture is the limited amount of media, [...]