In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines.
Following the successful fusion, clone selection and weaning from media supplements ten candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well).
The cell culture supernatant was purified with protein A. The objective here was to obtain initial quantities of purified antibodies for comparison purposes rather than to try and optimize the culture conditions. G-Rex is the perfect device for the development monoclonal antibody since each well acts as an individual “mini-bioreactor”, which is self-sustained due to G-Rex’s gas exchange membrane technology.
Simple, easy and fast development of monoclonal antibody
G-Rex Plates enable large numbers of any type of non-attachment cell to be grown to high numbers within a few days: hybridomas, THP-1, CHO, HeLa, SF9 etc.
In a G-Rex 6 Plate 5x10e6 cells can be expanded to between 200 – 400 x10e6 within around 10 days with only one exchange of medium. Hybridomas can produce high-titer antibody in useful amounts after 10 to 14 days.
No passaging required
A gas permeable silicone membrane at the base of each well ensures efficient oxygenation during cell expansion. This results in exponential growth of cells limited only by the amount and type of media in the well. Passaging is no longer required and high cell numbers are achieved with minimal effort.
Saves incubator space
G-Rex Plates free up space for other work in an incubator so can make a valuable contribution to the efficiency and productivity of many cell culture labs.