It has been shown (1) that iPSC derived multi-tissue organoids can be grown for up to 30 weeks in G-Rex bioreactors which provide a continuous exchange of gas at the cell/tissue interface via a basal gas-exchange membrane. Little routine maintenance is required apart from refreshing media every few days. G-Rex culture systems are produced to GMP grade by WilsonWolf Manufacturing LLC. and represent the gold-standard culture method for the rapid expansion of T-Cells destined for CAR-T therapy, for example (2).
G-Rex technology is available in research tool format : 6- and 24-well multi-well plates and in a 1 Litre bioreactor flask (EZ-Flask). See product page.
(1) Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids : https://doi.org/10.1101/2021.09.21.461213
(2) The beneficial effects of a gas-permeable flask for expansion of Tumor-infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity : https://doi.org/10.1080/2162402X.2015.1057386