Mass production of
uniform spheroids / EBs

Thousands of uniform spheroids per plate
Easy media changes
Compatible with standard imaging equipment
Pre-coated with anti-adhesive

The round-bottomed microwells in SP5D plates enable tight size- and shape-control of spheroid formation. This is key to establishing uniform signalling gradients around the spheroid core. This technology provides standardized, simultaneous production of a large number of spheroids on a single plate with minimal pipetting. Various plate formats are available (25, 750 and 3364 spheroids per well, see below.). Each of these plates allows for easy media changes to enable long term maintenance if required.

2400 spheroids per plate

25 spheroid replicates per well in 96-well format plate

NEW : 25 spheroid replicates per well with easy media changes and imaging. Provided in 96-well format with 12 removable strips of 8 wells. The SP5D 96w format plate (KDK096) offers greater statistical validity of results with less effort. Round-bottom microwells for optimal cell aggregation process.

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KDK096 SP5D 96-Well Plate (25 microwells per well)

9000 spheroids per plate

750 spheroids per well in 12 wells

A spheroid plate with round-bottomed microwells. The SP5D 24 well format plate (KDK024) has 24 wells with 750 spheroids per well in 2 rows, with 2 rows for 2D culture. Maximum spheroid diameter 150µm.

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KDK024 SP5D 24-Well Plate (750 microwells per macrowell)

20,184 spheroids per plate

3364 spheroids per well in 6-well format plate

NEW : A spheroid plate with microwells for easy scale-up of 3D culture. The SP5D 6-well plate with square wells (KDK006) is used to produce 3364 uniform spheroids per well and a total of 20184 spheroids per plate. Maximum spheroid diameter 150µm.

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KDK006 SP5D 6-Well Plate (3364 microwells per macrowell)

Technical advantages of SP5D plates

Rounded bottom of microwell for optimal cell aggregation into uniform spheroids

No requirement to do an anti-adhesive pre-coating

Produce many spheroids per pipetting step

Multiple spheroids in a well share the same media

Easy to change media

Patented design of SP5D plates

The design of the microscopic wells with a hemispherical base is optimised to drive the rapid clusturing of cells into compact, rounded spheroids. A unique nanocoating prevents cells from adhering to the surface. Consistently round spheroids are formed, fostering more natural gradient profiles and communication between cells.

The SP5D platform is ideal for consistently generating very large numbers of spheroids per plate boosting the statistical relevance of data from patient derived tumour samples. The platform is also ideal for generating pseudo-islets and spheroids for tissue engineering research.

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