FiberCell Hollow Fibre Cartridges
FiberCell Systems hollow fibre cartridges are supplied in 3 sizes, with various hollow fibre materials available. If you can tell us about your objective we will advise on the best options. Each cartridge includes a built-in gas exchange and pumping mechanism that integrates with the DUET pump. Cartridges are gamma-irradiated, single-use devices. Luer fittings facilitate coupling of cartridge to a media bottle via an autoclavable cap as well as inoculation/harvesting from the cell compartment usinb luer syringes, operations conducted in a laminar flow cabinet. The cartridge + media bottle are easily removed from and replaced back into the DUET pump which remains in the incubator.
| Cartridge/Flowpath Specifications | ||||||||
|---|---|---|---|---|---|---|---|---|
| Catalog No. | Size | Surface Area | Fibre Type | Packing Density | ECS Vol | MWCO 50% | Max. Cell# | Oxygenator |
| C2025 | Small | 80 cm2 | PVDF | 39% | 3.9 mL | 0.1 µm | 1E+8 | 1.5 m |
| C2025D | Small | 450 cm2 | High flux PS | 50% | 3.2 mL | 20 kD | 1E+8 | .65 m |
| C2025F | Small | 450 cm2 | High flux PS | 50% | 3.2 mL | 5 kD | 1E+8 | .65 m |
| C7025 | Small | 80 cm2 | High flux PVDF | 42% | 3.7 mL | 0.03 µM | 1E+8 | .65 m |
| C2008 | Medium | 4000 cm2 | Low flux PS | 50% | 20 mL | 5 kD | 1E+9 | 4 m |
| C2011 | Medium | 4000 cm2 | High flux PS | 50% | 20 mL | 20 kD | 1E+9 | 4 m |
| C5011 | Medium | 4000 cm2 | High flux PS | 50% | 20 mL | 20 kD | 2E+9 | 6.1 m |
| C7011 | Medium | 880cm2 | High flux PVDF | 50% | 24 mL | 0.03 µm | NA | 0.65 m |
| C2003 | Large | 1.2 m2 | Low flux PS | 50% | 70 mL | 5 kD | 5E+10 | 6.1 m |
| C2018 | Large | 1.2 m2 | High flux PS | 50% | 70 mL | 20 kD | 5E+10 | 6.1 m |
Cat # C2025D – 20 kD polysulfone
20 kD MWCO polysulfone fibre with 450 cm2 of surface area. Only one wrap of oxygenation tubing to reduce system dead volume. Popular application – culture of primary MSCs for production of extracellular vesicles and evaluation studies prior to scale-up (download protocol for MSC culture in hollow fibre bioreactor).
Cat # C2025 – SPECIAL 0.1 µm PVDF fibre (no wetting agent)
0.1 µm pore size for highest exchange rates but without protein retention. 80 cm2 outer surface area. PVDF fiber allow for the attachment of matrix proteins, cytokines and antibodies. Mentioned in several publications for endothelial cell culture under shear and various types of primary cell culture. Requires wetting with alcohol.
Cat # C7025 – 0.03 µm PVDF fibre
NEW PRODUCT 0.03 µm pore size for excellent exchange rates and easy sampling. 80 cm2 outer surface area.
Cat # C2008 – 5 kD polysulfone
Low MWCO (5 kd @ 50%) hydrophilic fibre for trapping smaller molecules. Suggested for recombinant proteins between 25 kd and 100 kd. Appropriate for suspension cell lines including CHO, HeLa and 293. Can support up to 1e9 cells and produce 100 µg/mL or more of recombinant protein in 20 mL ECS.
Cat # C2011 – 20 kD polysulfone
High MWCO (20 kd @ 50%) hydrophilic fibre for trapping larger recombinant proteins (120 kd on up depending upon glycosylation) hybridoma culture and lymphocyte culture. 20 kd MWCO allows TGF beta and TNF alpha to diffuse away while retaining antibodies. Can support up to 10e9 cells and produce 1-20 mg of a recombinant protein or 5-100 mg of monoclonal antibody every two days.
Cat # C5011 – 20 kD polysulfone for high output mAb production
The scale-up of antibody production has just been made easier. The C5011 cartridge can be managed in the same manner as the C2011 but with 2X greater productivity and twice the antibody concentration. The C5011 is the same cartridge as the C2011 but with more than twice the oxygenation capacity.
Cat # C7011 – PVDF fibre cartridge 0.03µm pore size rating
NEW – fewer fibres than the polysulfone cartridges with thicker microporous fibres having a very tight pore size rating and excellent gross filtration rate.
Cat # C2003 – 5 kD polysulfone
Low MWCO (5 kD @ 50%) hydrophilic fiber for trapping smaller molecules. Suggested for recombinant proteins between 25 kD – 100 kD.
Cat # C2018 – 20 kD polysulfone
High MWCO (20 kD @ 50%) hydrophilic fiber for trapping larger recombinant proteins (100 kD or larger). Can support up to 5×1010 cells and produce 5-20 mg of protein every day in a volume of 60 mL. Appropriate for adherent or suspension cell lines including CHO, HeLa, BHK and 293 cells.
FiberCell® Systems offers three types of fibers: polysulfone, cellulosic, and PVDF fibers. Molecular weight cut-offs (MWCO): 5 kD, 20 kD and pore size ratings of 0.1 µm and 0.03µm for PVDF microporous fibers.
Polysulfone fibres
The hydrophilic polysulfonefiber is specifically selected for good cell culture performance. Both the 5 kd MWCO and 20 kd MWCO fibers have ten times the gross filtration rate of the equivalent cellulosic fiber for rapid nutrient and waste exchange. This means that better cell growth and higher fidelity of protein production is possible compared to cellulosic fibres. Any cell type that will attach to plastic or grow in suspension culture should grow well in the polysulfone cartridge.
Cellulosic fibres
Not recommended for mammalian cell culture however these fibres are fine for use in antibiotic PK/PD applications.
0.1 µm PVDF fibre
Available only for the small cartridge module. This fibre allows protein matrices, antibodies and cytokines to be bound to the surface. Coating with a matrix is necessary prior to inoculation with cells. Use for primary cell types where you wish to control the surface matrix that the cells interact with and also for the growth of endothelial cells on the inside of the fibre.
0.03 µm PVDF fibre
Available for Small and Medium cartridges sizes. New product.
Molecular weight cut-off (MWCO)
FiberCell® Systems defines molecular weight cut offs at the 50% retention mark. “Useful retention” is the size of protein that will be retained at>=90%. Retention depends upon the size, shape, post-translational modifications and stokes radius of the protein, not just the molecular weight. For the 5 kD MWCO fibre the useful retention is>=20 kD and it should be used for proteins in the range of 20 kD on up to about 140 kD. For larger proteins, the 20 kD MWCO fiber should be used.
Cartridge Module Size
FiberCell® Systems cartridges are compact yet powerful cell culture tools. The medium sized C2011 with a surface area of 4,000 cm2 and a volume of only 20 mL has been shown to out-produce a 10 liter bag type bioreactor by a significant degree.
For recombinant protein production with CHO, HEK293…etc., select a cartridge size based on the amount you wish to collect. The larger cartridges should be used when 500 mg or more of a recombinant protein is desired.
For hybridoma culture always usea 20 kd MWCO medium-size cartridges, either C2011 or C5011.The 20 kd MWCO allowsinhibitory TGF beta to diffuse away. The scale-up option for monoclonal antibody production is the C5011 cartridge module which is optimized for highest output from hybridomas within the space of an incubator.