FiberCell® Systems offers three types of fibers: polysulfone, cellulosic, and PVDF fibers. We also offer three different molecular weight cut-offs (MWCO): 5 kD, 20 kD, and .1 micron.
The hydrophilic polysulfonefiber is specifically selected for good cell culture performance. Both the 5 kd MWCO and 20 kd MWCO fibers have ten times the gross filtration rate of the equivalent cellulosic fiber for rapid nutrient and waste exchange. This means that better cell growth and higher fidelity of protein production is possible compared to cellulosic fibres. Any cell type that will attach to plastic or grow in suspension culture should grow well in the polysulfone cartridge.
Not recommended for mammalian cell culture however these fibres are fine for use in antibiotic PK/PD applications.
Available only for the small cartridge module. This fibre allows protein matrices, antibodies and cytokines to be bound to the surface. Coating with a matrix is necessary prior to inoculation with cells. Use for primary cell types where you wish to control the surface matrix that the cells interact with and also for the growth of endothelial cells on the inside of the fibre.
Molecular weight cut-off (MWCO)
FiberCell® Systems defines molecular weight cut offs at the 50% retention mark. “Useful retention” is the size of protein that will be retained at>=90%. Retention depends upon the size, shape, post-translational modifications and stokes radius of the protein, not just the molecular weight. For the 5 kD MWCO fibre the useful retention is>=20 kD and it should be used for proteins in the range of 20 kD on up to about 140 kD. For larger proteins, the 20 kD MWCO fiber should be used.
FiberCell® Systems cartridges are powerful cell culture tools and their small size can be misleading. The medium sized C2011 with a surface area of 4,000 cm2 and a volume of only 20 mL has been shown to out-produce a 10 liter bag type bioreactor by a significant degree.
For recombinant protein production with CHO, HEK293…etc., select a cartridge size based on the amount you wish to collect. The larger cartridges should be used when 500 mg or more of a recombinant protein is desired.
For hybridoma culture always usea 20 kd MWCO medium-size cartridges, either C2011 or C5011.The 20 kd MWCO allowsinhibitory TGF beta to diffuse away. The scale-up option for monoclonal antibody production is the C5011 cartridge module which is optimized for highest output from hybridomas within the space of an incubator.