How much product yield can you get from a hollow fibre bioreactor?
Example: a hybridoma that yields 20µg/ml mAb in T-Flask would produce around 0.5 grams per month in around 400ml total harvest volume when using hollow fibre cartridge Medium Size Cat.# C2011, 20ml and double that amount in Cat.# C5011.
A single DUET pump can support 2 hollow fibre cartridge modules. Scale up with Large cartridges or scale out with more pumps. Each cartridge requires around 15 minutes of maintenance per day in the laminar flow cabinet.
Fits on shelf of a standard C02 incubator.
20ml and 70ml cartridges (20 ml shown)
Multiple cartridge set-up.
Multiple grams per month at high concentration.
Estimate your hollow fibre advantage for productions from hybridoma or stably expressing cells :
If the yield of mAb from your hybridoma cells in static flask culture is: Y µg/ml , then…
| mAb from hybridoma – weekly production rates | ||||||||
|---|---|---|---|---|---|---|---|---|
| 180L Incubator Capacity Required | DUET Pumps | Cartridges | Amount in mg | Total Weekly Harvest Vol. | ||||
| Single shelf | 1 | 1 x C2011 | 6Y mg | 60 ml | ||||
| Single shelf | 1 | 1 x C5011 | 12Y mg | 120 ml | ||||
Typical weekly production for stably expressing CHO, HEK, BHK cells…
| Recombinant protein – weekly production rates | ||||||||
|---|---|---|---|---|---|---|---|---|
| 180L Incubator Capacity Required | DUET Pumps | Cartridges | Amount in mg | Total Weekly Harvest Vol. | ||||
| 1 | 1 | 1 x C2011 | 10 mg | 100 ml | ||||
| 1 | 1 | 1 x C2018 | 40 to 50 mg | 300 ml | ||||
FiberCell Systems Inc., MD, USA, was established in year 2000.
Recombinant proteins, mAbs, vaccines – publications list
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