The left hand lanes of the above SDS gels show diluted raw supernatant. The media used was DMEM+CDM-HD + Glutamine 2 mM (1/100 Gibco 25030 200mM)+ PenStrep 1/100 (Gibco 15140). CDM-HD is recommended by FiberCell Systems for use with their bioreactor. With this system, U-CyTech has been able to simplify the purification procedure:
“… purification after culture is now shortened and improved. The concentration step, ultrafiltration, is not necessary with the Fibercell (We used to have yield loss during concentration). Now we only perform an ammonium sulfate precipitation and a chromatography step. The ion chromatography is more efficient because after AS precipitation the Fibercell material is much cleaner than from the roller bottles after AS precipitation.” – U-Cytech
DUET pump with 2 independent C2011 hollow fibre cartridges installed. Reservoir caps A1008, fit Costar 45mm neck autoclavable bottles.
Read more about using the FiberCell System for hollow fiber cell culture and monoclonal antibody production using the recommended CDM-HD media.