How Does a Hollow Fibre Bioreactor Work?
A hollow fibre bioreactor is a capillarized 3D culture system for continuous culture of a very large number of cells at high density. This enables periodic harvesting of concentrated cell-secreted products or the possibility to establish meaningful 3D in vitro models. Hollow fibre technology is now accessible at lab incubator scale in the form of simplified single-use cartridge modules from FiberCell Systems Inc.. For over 15 years FiberCell Systems have been making it easy and affordable for any lab to scale up from laborious culture of cells in traditional systems and profit from the multiple benefits of high density hollow fibre culture. Many academic and commercial customer references are available. (Image Right: Duet pump in a lab incubator with a 20ml cartridge module for maintaining up to 2E+9 cells in continuous culture).
Stylized schematic showing 3 fibres only
A FiberCell® cartridge opened
at one end to show fibres
A hollow fibre cartridge contains a bundle of many hundreds of porous, protein-retentive hollow fibre filters made of polysulfone. These high flux fibres allow cell densities to exceed 1E+8 cells per ml. FiberCell modules have fibres with 5 Kd or 20 Kd molecular weight cut-off (note: 50% retentive for a globular non-glycosylated protein at the rated MWCO).