CDM-HD Serum Replacement is designed for use in 2-compartment high density mammalian culture systems where cell densities exceed 1E+7 cells/ml. Under these conditions cell-secreted conditioning factors play a significant role in supporting the culture. Serum can therefore usually be completely replaced with CDM-HD. This simplified media is a mix of low molecular weight compounds that is chemically defined and consistent lot-to-lot as well as being very economical.
CDM-HD Serum replacement powder enables the cost, risks and lot-testing associated with serum to be eliminated. In the FiberCell bioreactor most types of cells require no adaptation period when switching over from serum to CDM-HD. Media produced using CDM-HD is protein-free and so greatly facilitates the purification of mAbs. Also, it is ideal for EV / exosome production with HEK293 and many other types of cells. It is a cGMP compliant product.
Problems with using serum
Because of undefined composition, risk of contaminations, cost factors and also animal welfare considerations, the switch to serum free alternatives is promoted by regulatory authorities, industry and academics in general. Serum is an ill-defined mixture and often contains adventitious agents and by-products like bacterial endotoxins and other immunogenic contaminants. Furthermore batch qualities vary and the costs can be high. Sera are a major source of viral contaminants which often do not produce cytopathic effects or morphological changes but once present are almost impossible to remove from cultures.
Some important advantages in the use of totally defined media are the avoidance of risks of contamination and immunogenic stimuli and lot to lot consistency Possible contaminants include viruses, prions and mycoplasma which highly impair pharmacologic use of culture derived products. By contrast, a totally defined, animal component free, protein free medium will not contain any proteins nor any materials derived from animals. Purification of the desired secreted product is facilitated and regulatory compliance is made much simpler.