Chemically defined serum replacement – specifically for high cell density culture

CDM-HD Chemically-Defined Serum Replacement is designed for use in 2-compartment high density mammalian culture systems where cell densities exceed 1E+7 cells/ml. Under these conditions cell-secreted conditioning factors play a significant role in supporting the culture. Serum can therefore usually be completely replaced with CDM-HD. This simplified media is a mix of low molecular weight compounds that is chemically defined and consistent lot-to-lot as well as being very economical.

CDM-HD enables the cost, risks and lot-testing associated with serum to be eliminated. In the FiberCell bioreactor most types of cells require no adaptation period when switching over from serum to CDM-HD. Media produced using CDM-HD is protein-free and so greatly facilitates the purification of mAbs. Also, it is ideal for EV / exosome production with HEK293 and many other types of cells. It is a cGMP compliant product.

Example uses of CDM-HD serum substitute

Single step switch to serum free conditions

It takes a few days for the contaminating serum proteins to be cleared once the switch to CDM-HD has been made. Afterwards the target protein is usually the dominant protein in the unpurified supernatant.

Data courtesy of: Dr. Erin Bromage c/o the
US Veterinary Immune Reagents Network

Example uses of CDM-HD

MAb harvesting – 2 different MAbs

Secreted products such as monoclonal antibodies and recombinant proteins are free of contaminating proteins from the medium and can be purified using simplified protocols, increasing net yield in many cases.

Example use of CDM-HD

Antibody from the FiberCell HFBR was very clean and properly folded.

Lane 1: control (3 ug)
Lanes 2, 3 and 4: unpurified