Estimate your hollow fibre advantage, below:

If the yield of your monoclonal antibody in flasks = Y µg per ml, see table below for approx. total output per month.

Example: Scaling-up mAb production
Incubator Capacity DUET Pumps Cartridges Amount/ month (µg) Vol
180L 1 shelf 1 1 x C2011 12000Y to 24000Y   in a volume of… 0.32 L
180L 1 shelf 1 1 x C5011 up to 48000Y  in a volume of… 0.32 L

If the yield of your rec. protein in flasks = Y µg per ml, see table below for approx. total output per month.

Example: Scaling-up recombinant protein – CHO cells
Incubator Capacity DUET Pumps Cartridges Amount / month Vol.
180L 1 shelf 1 1 x C2011 24000Y in a volume of…   0.45 L
180L 1 shelf 1 1 x C2018 72000Y in a volume of…   1.2 L

A single DUET pump can support 2 hollow fibre cartridge modules. Different cartridges are available with different capacities. More pumps and cartridges can be added as required. Each cartridge requires around 15 minutes of maintenance per day in the laminar flow cabinet. The Duet pump is designed to run continuously for years without maintenance.

Fits on shelf of a standard C02 incubator.
20ml and 70ml cartridges (20 ml shown)

Large scale hollow fibre bioreactor set-up

Multiple cartridge set-up.
Grams per month.

 

Lab Scale Hollow Fiber Cell Culture

FiberCell Systems Inc., MD, USA, was established in year 2000.

Recombinant proteins, mAbs, vaccines – publications list