FiberCell® Hollow Fibre Cartridge Modules

FiberCell Hollow Fibre Cartridge Modules come complete with oxygenation loop in a tidy unit along with all connections ready to clip together to a FiberCell reservoir cap to create a circuit for media recirculation. The design permits easy installation and removal from the Duet Pump enabling the user to unclip or re-insert the module while the pump is running.

These hollow fibre modules are suitable for culturing both adherent and suspension cells.

Small Size Cartridge

Activated PS+ Fibercell Cartridge  0.1µm

Activated fibre for attachment of matrix proteins, cytokines and antibodies followed by culture of cells on matrix. The PS+ fibre has a unique chemistry that allows protein matrices, antibodies and cytokines to be bound to both lumen and external surfaces. This opens the possibility for many innovative applications. Please enquire.

Applications

Primary cell culture with cell attachment to your chosen surface matrix

Endothelial cell culture under flow

Hepatocyte culture

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C2025 Small 75 cm2 activated PS+ 30% 2.5 ml 0.1 µm 108

Medium Size Cartridges

Medium Polysulfone Fibercell Cartridge  5kd

Low MWCO hydrophilic PS fibre cartridge. Smaller secreted molecules are efficiently retained for periodic harvesting from the extra-capillary space of the cartridge. Cell densities can be maintained at around 1-2 x 108 cells/ml. Option: replace serum with CDM-HD for harvests free of extraneous protein.

Applications

Production of rec. proteins and other secreted products
(MW 25 kd to 100 kd.)

Daily harvest: 100 µg/mL or more rec. protein in 20 ml volume

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C2008 Medium 3000 cm2 low flux PS 50% 20 ml 5 kd 109

Medium Polysulfone Fibercell Cartridge  20kd

High MWCO hydrophilic PS fibre cartridge. Will efficiently retain larger recombinant proteins (100 kd or larger) for routine harvesting from the extra-capillary space of the cartridge over weeks or months. Ideal for hybridoma culture at high density since small inhibitory cytokines are continuously flushed away. Cell density maintained around 1-2 x 108/ml. Option: replace serum with CDM-HD for harvests free of extraneous protein.

(This cartridge can out-produce a 10 liter bag type bioreactor by a significant degree.)

Applications

MAb production – hybridoma culture

5 – 50 mg MAb every 2 days in 20ml volume

Protein production (MW 120kd or more)

Exosome production

2 compartment PK/PD model

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C2011 Medium 3000 cm2 high flux PS 50% 20 ml 20 kd 109

HI OUTPUT MAb Cartridge  20kd   NEW

For scaling-up MAb production.

Same as C2011 cartridge but with more than twice the oxygenation capacity to give 2X greater antibody  harvest concentration and greater productivity at very little extra cost per cartridge. An excellent scale-up solution for experienced users of the standard 20 kd cartridge, C2011.

Applications

MAb production – hybridoma culture

Double the productivity of C2011

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C5011 Medium 3000 cm2 high flux PS 50% 20 ml 20 kd 2 x 109

Medium Cellulosic Fibercell Cartridge  10 kd

Cellulosic hollow-fibre cartridges are recommended for specific applications, please enquire.

Applications

2 compartment PK/PD model: for highly polar compounds only.

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C3008 Medium 2000 cm2 Cellulosic 38% 12 ml 10 kd 109

Large Cartridges

Large Polysulfone Fibercell Cartridge  5kd

Low MWCO hydrophilic PS fibre for efficiently trapping and harvesting smaller secreted products 25 – 100 kd. : increased fibre surface area represents approx. 10X scale up from C2008 with relatively little extra cost. High density culture conditions permit use of the recommended protein-free serum substitute CDM-HD.

Applications

Recombinant protein production (MW 25 kd and 100 kd.)

5 to 20 mg daily in 60ml volume

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C2003 Large 1.2 m2 low flux PS 50% 70 ml 5 kd 5 x 1010

Large Polysulfone Fibercell Cartridge  20kd

High MWCO hydrophilic PS fibre for efficient trapping and recovery of larger recombinant protein products (100 kd or larger). Can support up to 5×1010 cells and produce 5-20 mg of protein every day in a volume of 60 mL. For adherent or suspension cell lines including CHO, HeLa, BHK and 293 cells.

Applications

Rec. protein production (MW 120kd or more)

5 to 20 mg daily in 60ml volume

Cat. # Size Surface Area Fibre Type Packing Density ECS Vol MWCO 50% Max. Cell#
C2018 Large 1.2 m2 high flux PS 50% 70 ml 20 kd 5 x 1010

Request Quotation

CLICK HERE