CDM-HD Chemically Defined Medium (Powder)
CDM-HD Chemically Defined Medium is an additive for basal DMEM media. It is a replacement for serum that is ADCF and protein-free. CDM-HD is designed specifically for use in the FiberCell hollow fibre bioreactor although it has also been shown to work very well in high density 2-compartment flasks.
Download the FiberCell CDM-HD brochure.
Advantages – avoids the uncertainty and costs associated with the use of serum and obviates the need for expensive sophisticated media.
Chemically defined medium for use in high density bioreactors
- Supplied as an easy to store powder
- Avoids the typical risks of using serum (viruses, prions and mycoplasma)
- No proteins, No animal-derived components, No detergents
- Single step adaptation for most cell lines and hybridomas
- Highly consistent lot-to-lot, stable at 4°C for 1 month once made up
- Gives highest bioreactor yields
Easier purification
CDM-HD chemically defined medium is a protein and surfactant-free substitute designed specifically for high density cell culture in the FiberCell® hollow fibre bioreactor. Using CDM-HD means simplified purification protocols and increased net yield.
How is it used?
CDM-HD is provided as a pack of dry powder to be made up to 1 litre and sterile-filtered to be used just like serum – at a concentration of 10% in DMEM/High, for example. DMEM is the preferred basal media for highest cell density. One pack containing 33.36g enables 10 litres of serum-free media to be produced at low cost.
Optimized for high cell density bioreactors
CDM-HD chemically defined medium is fully optimized to exploit the unique high density conditions in a hollow fibre bioreactor or in high density culture flasks. (Not for standard flasks or rollers). An adaptation period is not needed, you can replace serum with DMEM/10%CDM-HD once cells are growing at high density. CDM-HD contains no detergents or other cell membrane stabilizing agents that sometimes interfere with assays or precipitations [see composition table].
References:
Humanization of JAA-F11, a Highly Specific Anti-Thomsen-Friedenreich Pancarcinoma Antibody and In Vitro Efficacy Analysis: Tati, S. et al.; Neoplasia,2017 19:716-733 [Open Access]
Large-scale preparation of extracellular vesicles enriched with specific micro-RNA: Yoo, KW et al. ; Tissue Engineering. 2018 [abstract]
Efficient production and enhanced tumor delivery of engineered extracellular vesicles: Watson DC et al.; Biomaterials 105, 2016: 195-205 [open access]
Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag: Jayanthi S. et al.; Protein Expr Purif. 2014 Oct; 102: 76–84. [Open Access]
Ordering Information
| Cat. # | Size | Pack Size |
|---|---|---|
| CDM-HD | Chemically Defined Medium for High Density Cell Culture (enough to make 10 Litres media at 10% conc.) | 36 g |