CDM-HD is a protein and surfactant-free serum substitute designed specifically for high density cell culture in the FiberCell® hollow fibre bioreactor. Using CDM-HD means simplified purification protocols and increased net yield.
How is it used?
CDM-HD is provided as a pack of dry powder to be made up to 1 litre and sterile-filtered to be used just like serum – at a concentration of 10% in DMEM/High, for example. DMEM is the preferred basal media for highest cell density. One pack containing 33.36g enables 10 litres of serum-free media to be produced at low cost.
Optimized for high cell density bioreactors
CDM-HD offers a serum substitute fully optimized to exploit the unique high density conditions in a FiberCell bioreactor or in high density culture flasks. (Not for standard flasks or rollers). An adaptation period is not needed, you can replace serum with DMEM/10%CDM-HD once cells are growing at high density. CDM-HD contains no detergents or other cell membrane stabilizing agents that sometimes interfere with assays or precipitations [see composition table].
Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag: Jayanthi S. et al.; Protein Expr Purif. 2014 Oct; 102: 76–84. [Open Access]
|Cat. # ||Size ||Pack Size |
|CDM-HD ||Serum Substitute for High Density Cell Culture (enough to make 10 Litres media at 10% conc) || 36 g |