Photos of cells growing in hollow fibre bioreactor
MSCs growing as 3D nodules in 20ml hollow fibre bioreactor
Fibres are approx 200µm in diameter. The distance between adjacent fibres is also about 200µm. Primary MSCs don’t proliferate very much, if at all, in HFBR culture.
Prostate cancer cell line – Medium 20ml hollow fibre bioreactor
MSCs – Small 3.5 ml hollow fibre bioreactor
IVECAT Research Group
Innovation in Vesicles and Cells for Application in Therapy
HEK293 cells – Medium 20ml hollow fibre bioreactor
Once cells in Medium cartridge are consuming >= 1g/day of glucose then CDM-HD chemically defined serum replacement can be used
Hybridomas oozing out of an opened hollow fibre bioreactor cartridge
Normally you wouldn’t open a cartridge like this! It was done just to show how hybridoma density can easily exceed 1E+9 in a HFBR.
Close-up shot of MSC spheroid in hollow fibre bioreactor
Difi cells after 6 months culture
Cartridge was sectioned after 6 months of culture at high cell density