Fibres are approx 200µm in diameter. The distance between adjacent fibres is also about 200µm. Primary MSCs don’t proliferate very much, if at all, in HFBR culture.
IVECAT Research Group Innovation in Vesicles and Cells for Application in Therapy
Once cells in Medium cartridge are consuming >= 1g/day of glucose then CDM-HD chemically defined serum replacement can be used
Recominant protein production
Normally you wouldn’t open a cartridge like this! It was done just to show how hybridoma density can easily exceed 1E+9 in a HFBR.
Cartridge was sectioned after 6 months of culture at high cell density
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