Cell types successfully cultured in hollow fibre bioreactors (not exhaustive)

143-B, A375, A431, A549, B16-F0, BHK, CaCo-2, CD4+, CEM, CHO, GL261, H9, HCT-8, HEK293, HeLa-S3, HuCCA-1, Huh7.5, Hybridoma, JEG-7, L-929, LoVo, MDCK, MRC5, MSCs, NPC-TW04, OLN-93, OL-B8, Oligodendroglia, S115, S2, SaOS-2, THP-1, VERO…

Hollow fiber graphic with cells

The vast majority of cell lines, attachment-dependent or suspension, grow well in a FiberCell hollow fibre bioreactor and after inoculation will expand to a density of around 10^8 cells per ml, usually within a week or ten days.

Coating of polysulfone hollow fibres with a protein matrix such as fibronectin can assist attachment, particularly in the case of primary cells. Proliferating cells may grow in 3D layers around the fibres or occasionally as nodules. Most cell lines do not require serum once growing at high density see: CDM-HD serum replacement.

Primary MSCs may not proliferate under hollow fibre bioreactor conditions but can be maintained for many weeks without differentiation. Primary MSCs can produce high purity EVs continuously, separated from recirculating FBS-containing media by the fibre molecular weight cut-off. Scientific publication references and a suggested protocol for MSC culture can be provided on request.

Please contact us to discuss your cell type and objectives.