A hollow fibre bioreactor (HFBR) provides a huge surface area for cell attachment in a low volume cartridge. The porous semi-permeable fibre walls have a molecular weight cutoff to retain both cells and cell-secreted products.
Cells are typically inoculated into the space around the fibres. Media recirculating on the luminal side not only provides the cells with nutrients and oxygen but simultaneously removes waste metabolites resulting in stable high density culture: cell numbers can reach as high as 2 billion in a scaleable 20ml bioreactor.
Multiple harvests of supernatant enriched in target product can be taken over many weeks. This is a proven system for generating significant amounts of concentrated EVs/exosomes, mAbs or recombinant proteins.
Using this approach any R&D lab can consider generating up to industrial scale quantities of their target product.