Hollow fibre cell culture
Continuous culture of up to 1e10 cells in a standard incubator

A compact FiberCell Systems Hollow Fibre bioreactor enables any lab to continuously culture large numbers of cells at high density inside a standard incubator. Cell density 100 x higher than T-Flask is possible. This is a proven system for generating significant amounts of concentrated EVs/exosomes, mAbs or recombinant proteins.
Cell-secreted products accumulate to high concentration

Stylized schematic showing 3 fibres only
Porous fibres with a molecular weight cutoff retain cells and cell-secreted products. Repeated sampling or collections of very concentrated products can be made over many weeks, as required.

FiberCell Systems Inc. (USA) established 2000.
Widely referenced in literature see publications lists.

Efficient scale up of cell culture
Highly concentrated secreted product
Biologically relevant 3-dimensional culture conditions
No shear stress on cells
Optimal for high fidelity protein expression
Avoids passaging
Reduces plasticware trash
Uses an economical chemically-defined media formulation
Suitable for suspension and anchorage-dependent cells
How to harvest from a FiberCell cartridge?
Harvesting is carried out in the laminar flow cabinet. The cartridge and media reservoir are in a closed loop which is easily decoupled from the Duet pump for transfer to the laminar flow cabinet. Using syringes, product-enriched supernatant can be periodically collected. The molecular weight cut-off of the hollow fibres traps and concentrates secreted products to give very high yields. Up to 100X greater product concentration than with flask culture is routinely possible from a hollow fibre bioreactor.

Simple disposable cartridge module

Further scale-up is easy

