Oxygenators for cell cultures, organs and bioreactors
Hollow fiber oxygenator range
For cell and organ cultures requiring high efficiency gas exchange check out this range of hollow fiber oxygenators with PMP membrane. Circulate media or blood through the extra-luminal space and gas through the fibers.
Available non-sterile or sterile.
Research use only.
Three sizes : fiber areas 160, 1900 and 2900 cm2.
Expand T-Cells and NK-Cells the easy way
G-Rex® technology for T-Cell Expansion
CAR-T researchers are discovering the advantages of G-Rex® culture devices. Easy and uninterrupted cell expansion is achieved with minimal user intervention thanks to the reliable gas exchange membrane technology built in to G-Rex® culture plates. Cell expansion protocols developed with G-Rex® culture plates directly scale up to clinical doses in larger G-Rex® bioreactors.
- Reduced labour
- Saves incubator space
Start here : https://www.kdbio.com/g-rex-cell-culture-plates/
A tumour spheroid culture platform designed specifically for functional precision medicine research
KDK096 SP5D 96-Well Plate (25 spheroids per well)
The Advantage of SP5D Patient-Derived Tumour Spheroids
Patient derived tumour spheroids provide a revolutionary tool that opens the way to designing cancer treatments tailored to the unique profile of individual patients. The new SP5D 96 well plate provides 12 strips of 8 wells with 25 microwells per well for spheroid formation, i.e. 2400 spheroids in total, increasing the number of replicates without extra pipetting. The removable well strips (200 spheroids) can be used individually.
It is the ideal product for accelerating the pace for time-critical cancer projects.
Optimising tumoroid culture for best results
In SP5D spheroid culture plates the microscopic wells are produced with a hemispherical base to drive the rapid clusturing of cells into 25 rounded spheroids per microwell. A unique nanocoating prevents cells from adhering to the surface. Consistent spheroids are formed, and in a single macrowell the spheroids share the media, fostering more natural communication between cells.
The new SP5D 96 Well platform is ideal for easily generating 25 spheroid replicates per well thereby boosting the statistical relevance of data from patient derived tumour samples.
How to use Sphericalplate 5D
Preparation
Prepare functional well with 0.1 mL of medium
Addition of Cells
Add 0.1 ml of single cell suspension
Cultivation
Incubate
Related product for precision oncology research
G-Rex Plates for Easy Expansion of T-Cells, NK-Cells…
KDBIO is now EU supplier for Kugelmeiers SP5D Plates
Produce up to 20,184 spheroids on a single culture plate
Product page with link to free-to-view scientific publication references here
Scaffold devices for long term culture in 3D
KDBIO (Europe) now supplies Gelacell Plates for 3D Culture
A variety of nanoporous synethetic and natural polymer scaffolds, including aligned fiber scaffolds, are now available in Europe through KDBIO. For more information and an overview of available references see : https://www.kdbio.com/gelacell-nanofiber-scaffolds-for-3d-cell-culture/
It has been shown (1) that iPSC derived multi-tissue organoids can be grown for up to 30 weeks in G-Rex bioreactors which provide a continuous exchange of gas at the cell/tissue interface via a basal gas-exchange membrane. Little routine maintenance is required apart from refreshing media every few days. G-Rex culture systems are produced to GMP grade by WilsonWolf Manufacturing LLC. and represent the gold-standard culture method for the rapid expansion of T-Cells destined for CAR-T therapy, for example (2).
G-Rex technology is available in research tool format : 6- and 24-well multi-well plates. See product page.
(1) Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids : https://doi.org/10.1101/2021.09.21.461213
(2) The beneficial effects of a gas-permeable flask for expansion of Tumor-infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity : https://doi.org/10.1080/2162402X.2015.1057386
Check out this new range of hollow fiber ultrafilters from KDBIO. Attractive prices.
Various sizes of hollow fiber cartridge, available sterile or non-sterile, for concentration/diafiltration of cytokines and larger proteins/viruses, and for cell perfusion experiments. Datasheets via links on product page.
Prepare 1 Litre or more of monoclonal antibody from hybridoma using EZ-Flask with serum free media
- Add 250ml serum free hybridoma media to EZ-Flask
- Inoculate with 10^7 hybridomas
- Wait 1 week without disturbing EZ-Flask, then add 250ml media
- Wait 1 week without disturbing EZ-Flask, then add 250ml media
- Wait 1 week without disturbing EZ-Flask, then add 250ml media
- Harvest high titre antibody during following week
How does this work exactly? Please contact us to find out: [email protected]
Product page : https://www.kdbio.com/ez-flask-for-hybridomas/
In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines.
Following the successful fusion, clone selection and weaning from media supplements, candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well).
The cell culture supernatant was purified with protein A. The objective here was to obtain initial quantities of purified antibodies for comparison purposes rather than to try and optimize the culture conditions. G-Rex is the perfect device for the development monoclonal antibody since each well acts as an individual “mini-bioreactor”, which is self-sustained due to G-Rex’s gas exchange membrane technology.
Simple, easy and fast development of monoclonal antibody
G-Rex Plates enable large numbers of any type of non-attachment cell to be grown to high numbers within a few days: hybridomas, THP-1, CHO, HeLa, SF9 etc.
In a G-Rex 6 Plate 5x10e6 cells can be expanded to between 200 – 400 x10e6 within around 10 days with only one exchange of medium. Hybridomas can produce high-titer antibody in useful amounts after 10 to 14 days.
No passaging required
A gas permeable silicone membrane at the base of each well ensures efficient oxygenation during cell expansion. This results in exponential growth of cells limited only by the amount and type of media in the well. Passaging is no longer required and high cell numbers are achieved with minimal effort.
Saves incubator space
G-Rex Plates free up space for other work in an incubator so can make a valuable contribution to the efficiency and productivity of many cell culture labs.