For laboratories wishing to produce large amounts of extracellular vesicles (exosomes) from MSCs using a hollow fibre bioreactor, FiberCell Systems has now published a preliminary protocol based on feedback from users in Europe and USA. If you need to quickly generate concentrated and pure EVs from MSCs this document is definitely a “must read”. It contains some eye-opening information, and opinion, about the key differences between continuous hollow fibre culture compared to traditional batch culture for EV productions.

Protocol for producing extracellular vesicles from MSCs