mAb production (hybridoma) in different systems

Monoclonal antibody production can be achieved using various culture systems.

Below you will find a comparative table showcasing different bioreactors, their features and the advantages they provide.

G-Rex 6 Well PlateEZ-FlaskFiberCell C2011
Batch or ContinuousBatchBatchContinuous
Harvest volume35 ml / well1 Litre20ml, 3X per week
Duration of production15 to 30 days15 – 30 daysTypically 2 to 3 months
Production range (mg)0.15 to 7.5 mg3 to 150 mgup to 600mg /month/cart.
mAb concentration rangeUp to 0.15mg/mlUp to 0.15mg/ml0.5 to 5 mg/ml
To scale up, use …EZ FlaskFiberCell C2011FiberCell C5011
Web page for more info…G-Rex PlatesEZ FlaskFiberCell System

KDBIO offers a range of scale-up options tailored to your monoclonal antibody production requierements.

Starting with the smallest device,  G-Rex is a compact and versatile plate containing 6 or 24 wells. Each well behaves like a “mini-bioreactor” and the gas permeable membrane at its base permits an efficient oxygenation of the culture.

Scaling up from there, you will find the EZ-Flask to be an extremely convenient culture system. It is a 1 litre flask, and once inoculated, it can be left untouched in the incubator for up to 30 days without any user intervention. This system has produced up to 180 mg/L of monoclonal antibody at its peak performance after 30 days.

For hundreds of mg up to grams of mAb, the FiberCell HollowFibre Bioreactor is hands down the most productive and concentrative system for monoclonal antibody production. The 20 KDa 2011 Cartridge allows the continuous culture of large numbers of cells at high density.  It’s a scalable production process that’s ideal for generating significant amounts of very concentrated EVs/exosomesmAbs  or recombinant proteins.