Monoclonal antibody production (hybridoma) in different systems

Monoclonal antibody production can be achieved using various culture systems. Depending on the yield you are looking for you might need a different type of system. KDBIO offers 3 scale-up cell culture systems from small batch quantities up to continuous gram production quantities.

Below you will find a comparative table showcasing different bioreactors, their features and the advantages they provide.

G-Rex 6 Well PlateEZ-FlaskFiberCell C2011
Batch or ContinuousBatchBatchContinuous
Harvest volume35 ml / well1 Litre20ml, 3X per week
Duration of production15 to 30 days15 – 30 daysTypically 2 to 3 months
Production range (mg)0.15 to 7.5 mg3 to 150 mgup to 600mg /month/cart.
mAb concentration rangeUp to 0.15mg/mlUp to 0.15mg/ml0.5 to 5 mg/ml
To scale up, use …EZ FlaskFiberCell C2011FiberCell C5011
Web page for more info…G-Rex PlatesEZ FlaskFiberCell System
1 page PDF guide on how to scale-up monoclonal antibody production

Discover our scale-up guide for monoclonal antibody production

KDBIO offers a range of scale-up options tailored to your monoclonal antibody production requierements.

Starting with the smallest device, G-Rex is a compact and versatile plate containing 6 or 24 wells. Each well behaves like a “mini-bioreactor” and the gas permeable membrane at its base permits an efficient oxygenation of the culture. Because of this technology, passaging is not required and the G-Rex Plates can be kept for long periods of time in the incubator without an user intervention.

Scaling up from there, you will find the EZ-Flask bioreactor to be an extremely convenient culture system. It is a 1 litre culture flask, and once inoculated, it can be left untouched in the incubator for up to 30 days without any user intervention or passaging. EZ-Flask works with the same gas exchange membrane technology as the G-Rex. This system has produced up to 180 mg/L of monoclonal antibody at its peak performance after 30 days. Its compact design is made to save significant amounts of space in the incubator compared to cumbersome T-Flasks.

For hundreds of mg up to grams of monoclonal antibody, the FiberCell HollowFibre Bioreactor is hands down the most productive and concentrative system for monoclonal antibody production. The 20 KDa 2011 Cartridge allows the continuous culture of large numbers of cells at high density, for long periods of time (up to 1 year). It is a scalable production process that’s ideal for generating significant amounts of very concentrated EVs/exosomes, mAbs or recombinant proteins.

Below you will find a video describing the major differences between all 3 cell culture systems for monoclonal antibody production.

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