Monoclonal antibody production (hybridoma) in different systems
Monoclonal antibody production can be achieved using various culture systems. Depending on the yield you are looking for you might need a different type of system. KDBIO offers 3 scale-up cell culture systems from small batch quantities up to continuous gram production quantities.
Below you will find a comparative table showcasing different bioreactors, their features and the advantages they provide.
|G-Rex 6 Well Plate||EZ-Flask||FiberCell C2011|
|Batch or Continuous||Batch||Batch||Continuous|
|Harvest volume||35 ml / well||1 Litre||20ml, 3X per week|
|Duration of production||15 to 30 days||15 – 30 days||Typically 2 to 3 months|
|Production range (mg)||0.15 to 7.5 mg||3 to 150 mg||up to 600mg /month/cart.|
|mAb concentration range||Up to 0.15mg/ml||Up to 0.15mg/ml||0.5 to 5 mg/ml|
|To scale up, use …||EZ Flask||FiberCell C2011||FiberCell C5011|
|Web page for more info…||G-Rex Plates||EZ Flask||FiberCell System|
Discover our scale-up guide for monoclonal antibody production
KDBIO offers a range of scale-up options tailored to your monoclonal antibody production requierements.
0 – 2 mg : Starting with the smallest device, G-Rex is a compact and versatile plate containing 6 or 24 wells. Each well behaves like a “mini-bioreactor” and the gas permeable membrane at its base permits an efficient oxygenation of the culture. Because of this technology, passaging is not required and the G-Rex Plates can be kept for long periods of time in the incubator without an user intervention.
0 – 150 mg : Scaling up from there, you will find the EZ-Flask bioreactor to be an extremely convenient culture system. It is a 1 litre culture flask, and once inoculated, it can be left untouched in the incubator for up to 30 days without any user intervention or passaging. EZ-Flask works with the same gas exchange membrane technology as the G-Rex. This system has produced up to 180 mg/L of monoclonal antibody at its peak performance after 30 days. Its compact design is made to save significant amounts of space in the incubator compared to cumbersome T-Flasks.
100 – 1000 mg or more : For hundreds of mg up to grams of monoclonal antibody, the FiberCell HollowFibre Bioreactor is hands down the most productive and efficient system for monoclonal antibody production. The continuous culture of > 1e9 cells at high density, for long periods of time (up to 1 year) is possible. It is a scalable production process that’s ideal for generating significant amounts of very concentrated EVs/exosomes, mAbs or recombinant proteins.
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Monoclonal antibody production comparison in G-Rex, EZ-Flask and FiberCell C2011 Cartridge.
Monoclonal antibody production (hybridoma) in different systems Monoclonal antibody production can be achieved using various culture systems. Depending on the yield you are looking for you might need a different type of system. KDBIO [...]
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Publications referring to FiberCell System
Access lists of relevant references / Extracellular vesicles production / Monoclonal antibodies production / Recombinant proteins production / Secretome from 3D cultures / Virus production