Development of monoclonal antibody using G-Rex Plates

In this example, here are the results of the development of monoclonal antibody for 10 newly developed monoclonal cell lines. Following the successful fusion, clone selection and weaning from media supplements, candidate clones were transfered to wells of G-Rex 6 Well Plates (35ml media per well, and 10cm2 gas exchange membrane per well). The cell [...]

By |2022-06-15T13:50:34+00:00August 19th, 2021|blog|0 Comments

EV Core Poster

Comparison of Production and Activity of Amniotic Fluid Stem Cell Extracellular Vesicles from 3D Hollow Fiber Bioreactor and 2D culture. ISEV2020 Poster Laura Perin et al. ;Introduction: EV clinical translation is limited by scale-up of EVs production. Hollow fiber bioreactors (HFBR) support culture of large numbers of cells at high densities. [...]

By |2022-03-23T12:37:49+00:00August 12th, 2021|blog|0 Comments

Scaling up production of Netrin-1 for structural biology, functional and biophysical studies

Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor Applied Microbiology and Biotechnology August 2021 https://doi.org/10.1007/s00253-021-11438-0 Aniel Moya-Torres et al. Key Points: Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 1.0.0.20 [...]

By |2022-03-23T12:37:49+00:00August 11th, 2021|blog|0 Comments

U-Cytech Switches to FiberCell Hollow Fiber cell culture for mAb production

U-CyTech biosciences switches from roller bottles to FiberCell Systems Hollow Fibre Bioreactor U-CyTech biosciences is based in Utrecht, the Netherlands, and produces a wide range of innovative cytokine ELISA, ELISPOT and Fluorospot systems (human, monkey, rodent). Productions of monoclonal antibody with hybridomas are now performed at U-CyTech’s Utrecht laboratory using the FiberCell Hollow Fibre Bioreactor [...]

By |2022-03-23T12:37:49+00:00June 15th, 2021|blog|0 Comments

Monoclonal antibody production comparision in G-Rex, EZ-Flask and FiberCell C2011 Cartridge.

Monoclonal antibody production (hybridoma) in different systems Monoclonal antibody production can be achieved using various culture systems. Depending on the yield you are looking for you might need a different type of system. KDBIO offers 3 scale-up cell culture systems from small batch quantities up to continuous gram production quantities. Below you will find [...]

By |2022-03-23T12:37:49+00:00June 14th, 2021|blog|0 Comments

New YouTube Channel

New YouTube Channel - Hollow fibre bioreactor technology and applications KDBIO has launched a Youtube channel with short videos to explain hollow fibre bioreactor technology and the advantages this offers for various applications. KDBIO YouTube Channel  

By |2022-03-23T12:37:50+00:00February 5th, 2021|blog|0 Comments

YouTube video about CDM-HD

CDM-HD : chemically-defined media for high density culture Once cells are growing at high density in a FiberCell bioreactor it is generally very easy to switch over to CDM-HD (DMEM/10%CMD-HD) which is ideal for generating cell-secreted products such as monoclonal antibodies or extracellular vesicles.

By |2022-03-23T12:37:50+00:00October 13th, 2020|blog|0 Comments

July 2020 publication refers to EV production in hollow fibre bioreactor

Osteosarcoma-Derived Extracellular Vesicles Induce Lung Fibroblast Reprogramming Alek Mazumdar et al., Dept. of Orthopedics, Balgrist University Hospital, Zurich, Switzerland; Int. J. Mol. Sci. 2020, 21, 5451; doi:10.3390/ijms21155451 "....our findings provide novel insights into the functional relevance of EV-mediated communication between osteosarcoma cells and normal pulmonary fibroblasts. We deliver strong evidence that osteosarcoma cell-derived EVs [...]

By |2021-02-04T08:35:13+00:00September 2nd, 2020|blog|0 Comments

In pursuit of biological specificity

Which culture method is best to generate reagents with native biological specificity? Cell culture methods vary a lot with respect to their ability to generate secreted proteins and antibodies that possess true biological specificity. Culture methodology is therefore a critical consideration for a manufacturer of serological test kits where excellent specificity is a priority. [...]

By |2021-02-04T08:35:13+00:00April 14th, 2020|blog|0 Comments
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