25 spheroid replicates per well for better data

SP5D 96-Well Plate (25 spheroids per well) Cat.# KDK096

Designed for personalized medicine research

The SP5D 96w format 3D culture plate is ideal for generating, maintaining, treating and analysing patient-derived tumour spheroids. It provides 25 spheroid replicates per well. Each well has a 5×5 array of 25 microwells and all the spheroids share the same media in the well. Only 4000 to 16000 cells per well are needed to generate 25 spheroids. The plate is provided as 12 x 8-well strips (for 200 spheroids) that can be removed individually from a standard size frame. This makes it very convenient for processing small amounts of material from different individuals.

Uniformity

Spheroid aggregation uniformity is enhanced by the round-bottom microwell design.

Imaging and histological analysis

The plate is compatible with standard imaging methods so there is no need to transfer spheroids for imaging. However it is easy to recover all 25 spheroids from a well, for histology. Also, it is easy to change media for longer-term culturing. The anti-adhesive treated microwells can accommodate spheroids up to 500µm diameter. The plate is compatible with standard imaging platforms.

Advantages of SP5D tumour spheroids compared to tumour organoids :

Easier to produce, maintain, and analyse

Faster method to obtain data with higher statistical validity

Very cost-effective

Optimising tumour spheroid culture for best results

In SP5D spheroid culture plates the microscopic wells are produced with a hemispherical base to drive the rapid clusturing of cells into 25 rounded spheroids per microwell. A unique nanocoating prevents cells from adhering to the surface. Consistent spheroids are formed, and in a single macrowell the spheroids share the media, fostering more natural communication between cells.

The new SP5D 96 Well platform is ideal for easily generating 25 spheroid replicates per well thereby boosting the statistical validity of data from patient derived tumour tissue.