control (3 ug)
Lanes 2, 3 and 4:
unpurified harvests from the cartridge on day 7, 9 and 12 (2µl)
Lane 5: 10X conc. supernatant from a T-flask using DMEM and 10% CDM-HD (FiberCell) (2µl).
Lane 6: T flask using DMEM and 10% serum (2µl)
Both SDS-PAGE gel and gel filtration analysis showed that the antibody from the hollow fiber bioreactor (HFB) was very clean and properly folded.
Concentration of supernatant from HFB:
Approx 2.5 mg/ml.