Hollow-fiber bioreactor production of extracellular vesicles from human bone marrow mesenchymal stromal cells yields nanovesicles that mirrors the immuno-modulatory antigenic signature of the producer cell: Gobin, J. et al. Stem Cell Research and Therapy(2021) 12:127 [open access]… “The hollow-fiber bioreactor from FiberCell Systems allows for seeding large amounts of adherent cells based on its hollow-fiber technology which consequently increases the cell seeding surface area (medium-sized cartridge offers 4000 cm2 of surface area). In turn, this configuration allows for a scale-up of continuous EV production sampled over time from the EV-rich cell-conditioned medium produced by millions of cells, thus allowing anticipated clinical doses to be achieved.”
Engineered extracellular vesicle decoy receptor-mediated modulation of the IL6 trans-signalling pathway in muscle Mariana Conceicao et al. [open access]… “For in vivo experiments, FiberCell hollow fiber bioreactors with 20 kDa cutoff hydrophilic fibers (KDBIO, Berstett, France) were used for large-scale production of MSC-derived EVs, according to manufacturer’s instructions.”
Hypoxia-induced tumor exosomes promote M2-like macrophage polarization of infiltrating myeloid cells and microRNA-mediated metabolic shift: Park, JE et al.; Oncogene (2019) [abstract].. “B16-F0, A375, A431, orA549 cells were inoculated into the extra-capillary space of each cartridge and allowed to attach to the fiber surface over 24 h. Cancer cells were maintained in DMEM supplemented with 5% Chemically Defined Medium for High Density cell culture (CDM-HD; Fibercell). CDM-HD, a chemically defined protein-free serum replacement, was used to avoid contamination of FBS-derived exosomes”. Author describes use of hollow fibre culture system and workflow of exosome isolation.
Efficient production and enhanced tumor delivery of engineered extracellular vesicles: Watson DC et al.; Biomaterials 105, 2016: 195-205 [open access]“…these data suggest that the use of hollow-fiber bioreactor, serum-free media [CDM-HD] and an optimized purification protocol represent a superior method to achieve high yield of purified EV (yields were >3 mg/week from a single lab-scale [20ml, Medium Size] culture cartridge)..”