Culture endothelial cells under shear stress

Pulsatile flow conditions for endothelial cells grown in artificial capillaries

The FiberCell Systems 0.1µm hollow fibre module (cat# C2025) enables the culture and study of endothelial cells exposed to pulsatile shear stress in an artificial capillary system. Under conditions that closely mimic the in vivo situation, endothelial cells spread out as an endothelium monolayer on the inner surface of the fibres with the formation of tight junctions between cells. The system can be used to study CLL cell transmigration, mechanotransduction effects, interactions with RBC’s etc.

  • Artificial capillary system with inner 0.1µm hollow fibre surface area equal to a T75 flask
  • EC’s grow to line the inner lumen
  • EC’s can be exposed to a shear stress of 0.5 to 25 dynes/cm2 (pulsatile flow)
  • Transendothelial migration of lymphocytes can be studied (see references)
  • Cells can be recovered for analysis
  • Approximately 100 micrograms of EC RNA can be isolated from inside fibres
Endothelial cells cultured in artificial capillary

Human coronary endothelial cells coating
the internal surface of a PS+ hollow fibre
– Guerkan Sengoelge,
Medical University of Vienna

CLL cells shown to transmigrate

Data generated by a UK lab using the FiberCell C2025 cartridge and Duet pulsatile pump showed that the system enables  a novel, dynamic, and tractable in vitro model of lymphocyte migration. Also the results confirmed that CD49d is a critical regulator of lymphocyte migration in CLL. See reference below.

Consistent and defined shear stress

The microprocessor controlled FiberCell Systems pump can be programmed to produce consistent and defined amounts of shear stress by regulating the flow of medium over the cells. This allows the study of endothelial cells in a more physiologic environment when compared to other methods.

Co-culturing endothelial cells with smooth muscle cells…etc.

The microporous nature of the fibres and the ability to control the extra-cellular matrix provides an ideal system for cellular co-cultivation with other cell types such as vascular smooth muscle or neuroglia.

Attachment of cells to internal walls of fibres

The FiberCell 0.1µm fibres must first be coated with extra-cellular matrix proteins (and/or cytokines and antibodies) to permit the attachment of endothelial cells to the interior wall of the fibre.

References:

Phenotype and immune function of lymph node and peripheral blood CLL cells are linked to transendothelial migration: Pasikowska, M. et al; Blood 2016 128:563-573

Development and characterization of a physiologically relevant model of lymphocyte migration in chronic lymphocytic leukemia: Walsby, E. et al.; Blood 2014 123:3607-3617 [open access]

PECAM-1 and caveolae form the mechanosensing complex necessary for NOX2 activation and angiogenic signaling with stopped flow in pulmonary endothelium: Noel,J. et al.; Am J Physiol Lung Cell Mol Physiol. 2013 305(11): L805–L818. [open access]

Endothelial Cell Capture of Heparin-Binding Growth Factors under Flow: Zhao, B. et al.; PLOS Computational Biology, 2010. [open access]

CAVEOLAE ARE AN ESSENTIAL COMPONENT OF THE PATHWAY FOR ENDOTHELIAL CELL SIGNALING ASSOCIATED WITH ABRUPT REDUCTION OF SHEAR STRESS: Milovanova, T. et al.; Biochim Biophys Acta. 2008 1783(10): 1866–1875. [open access]

Lung Ischemia: A Model for Endothelial Mechanotransduction: Chatterjee, S. et al.; Cell Biochem Biophys.2008; 52(3) [open access]

Starter package for endothelial cell culture under flow conditions

Cat.# Description
P3202 FiberCell® Systems Duet
C2025 Activated PS Hollow Fibre Cartridge, 75cm2, 0.1µm*
A1005 33mm Reservoir Cap
A1006 38mm Reservoir Cap

* The C2025 cartridge houses 20x 0.1µm fibres each with an internal diameter of 700μm and an active length of 10cm for a total surface area of 75cm2

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