Virus Production

Virus production in T-flasks or roller bottles means dedicating a lot of work, time and space whereas high density cell culture in a FiberCell Hollow Fibre Bioreactor can yield very high amounts of viral particles with less effort and cost. In addition this high density mode of cell culture can be completely serum free without the need for expensive specialty media.

Example: Adenovirus

When 293 cells are adapted to suspension culture, large quantities of adenoviral vectors can be produced in a single FiberCell® Systems cartridge. High cell density makes for rapid and uniform infection kinetics within the ECS of the cartridge. A medium size FiberCell C2011 cartridge (20ml) produced 1-3×10^13 virus particles, equivalent to around 20 x 850 cm2 roller bottles. This could be scaled up by 10 X without purchasing extra hardware.

  • Closed, biosafe system that fits in a standard incubator
  • Serum free medium
  • Precise control of pH for optimum infections
FiberCell set up for virus production
C2011 cartridge module

References:

Production of high-titer human influenza A virus with adherent and suspension MDCK cells cultured in a single-use hollow fiber bioreactor: Tapia, F. et al.; Vaccine 2014 Feb 12;32(8):1003-11 [Open Access]

Considerations in Scale-Up of Viral Vaccine Production: William G. Whitford and Alain Fairbank; BioProcess International 2011 September [PDF]

Antiviral pharmacodynamics in hollow fibre Bioreactors: McSharry, J. et al.; Antiviral Chemistry & Chemotherapy 2011; 21:183–192 [open access]

A Hollow fiber bioreactor for expanding HIV-1 in human lymphocytes used in preparing an inactivated vaccine candidate: Leong M, Bobbitt W, Vyas G, Biologicals, 2007 35 (4):227-233.

Advanced methods of adenovirus vector production for human gene therapy: roller
bottles, microcarriers, and hollow fibers: T. Isayeva, et al.; BioProcessing Journal 2003 75-81 [PDF]

Suggested starter package

Starter kit for virus production

Cat.# Description Qty
P3202 FiberCell® Systems Duet 1
C2011 High Flux PS Cartridge 20 kd (3000 cm2 – 20 ml)* 1
A – – – 33mm, 38mm or 45mm Reservoir Cap 1
CDM-HD Serum replacement 1

* Option: C2018 High Flux PS Cartridge 20 kd (1.2 m2 – 70 ml), providing 4 X more fibre surface area.

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