Laboratory hollow fibre bioreactor can be used for virus production
Virus production in T-flasks or roller bottles means dedicating a lot of work, time and space. Switching to high density cell culture in a FiberCell Hollow Fibre Bioreactor can yield very high amounts of viral particles with less effort and cost. In addition this high density mode of cell culture can be exploited to move to a simpler chemically defined media without the need for serum.
Production example: HCV
Peak HCV titer of 7.6log10 FFU/ml has been reported from Huh7.5 cultured at high density in a FiberCell Hollow Fibre Bioreactor under serum-free conditions. NGS analysis showed high genetic stability. Higher infectivity titers were observed in serum-free media compared to serum-containing high density culture. A 20ml bioreactor cartridge (cat.# C2011) was able to produce 1µg of HCV virus over 65 days of operation. 70ml cartridges are also available. See article in Nature Article.
Production example: Adenovirus
When 293 cells are adapted to suspension culture, large quantities of adenoviral vectors can be produced in a single FiberCell® Systems cartridge. High cell density makes for rapid and uniform infection kinetics within the ECS of the cartridge. A medium size FiberCell C2011 cartridge (20ml) produced 1-3×10^13 virus particles, equivalent to around 20 x 850 cm2 roller bottles. This could be scaled up by 10 X without purchasing extra hardware.