Virus Production

Efficiently produce high titer virus in your laboratory

Hollow fibre bioreactor for virus production
  • Closed culture system for biosafety
  • Fits in a standard incubator
  • Serum free medium
  • Precise control of pH for optimum infections

Laboratory hollow fibre bioreactor can be used for virus production

Virus production in T-flasks or roller bottles means dedicating a lot of work, time and space. Switching to high density cell culture in a FiberCell Hollow Fibre Bioreactor can yield very high amounts of viral particles with less effort and cost. In addition this high density mode of cell culture can be exploited to move to a simpler chemically defined media without the need for serum.

Production example: HCV

Peak HCV titer of 7.6log10 FFU/ml has been reported from Huh7.5 cultured at high density in a FiberCell Hollow Fibre Bioreactor under serum-free conditions. NGS analysis showed high genetic stability. Higher infectivity titers were observed in serum-free media compared to serum-containing high density culture. A 20ml bioreactor cartridge (cat.# C2011) was able to produce 1µg of HCV virus over 65 days of operation. 70ml cartridges are also available. See article in Nature Article.

Production example: Adenovirus

When 293 cells are adapted to suspension culture, large quantities of adenoviral vectors can be produced in a single FiberCell® Systems cartridge. High cell density makes for rapid and uniform infection kinetics within the ECS of the cartridge. A medium size FiberCell C2011 cartridge (20ml) produced 1-3×10^13 virus particles, equivalent to around 20 x 850 cm2 roller bottles. This could be scaled up by 10 X without purchasing extra hardware.

In vitro anti-viral PK/PD

The hollow fibre culture system has been used to model human PK for anti-virals and to take samples for analysis over long periods of operation [more…]

Monoclonal antibody production

The FiberCell System is ideal for any lab to undertake rapid production of monoclonal antibodies in the range 100 mg to grams [more…]

FiberCell set up for virus production
C2011 cartridge module

Suggested starter package – easily affordable

Starter kit for virus production

Cat.# Description Qty
P3202 FiberCell® Systems Duet 1
C2011 High Flux PS Cartridge 20 kd (3000 cm2 – 20 ml)* 1
A – – – 33mm, 38mm or 45mm Reservoir Cap 1
CDM-HD Serum replacement 1

* Option: C2018 High Flux PS Cartridge 20 kd (1.2 m2 – 70 ml), providing 4 X more fibre surface area.

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