Compact bioreactor for high-titre virus production

Hollow fibre bioreactor for virus production
  • Closed culture system for biosafety
  • Fits in a standard incubator
  • Serum free medium
  • Precise control of pH for optimum infections

 

Large amounts of enveloped virus have been produced at high titer using a hollow fibre bioreactor

Although lytic viruses have been produced in a hollow fibre bioreactor [1] this continuous mode of cell culture is much better adapted to production of enveloped viruses that bud off from the cell surface. Daily collections of small volumes of cell supernatant can be taken. In this way, HCV virus has been produced with Huh7.5 cells over weeks at remarkably high titer using a leading to large total amounts [2].

[1] Advanced methods of adenovirus vector production for human gene therapy: roller bottles, microcarriers, and hollow fibers: T. Isayeva, et al.; BioProcessing Journal 2003 75-81 [PDF]

[2] High density Huh7.5 cell hollow fiber bioreactor culture for high-yield production of hepatitis C virus and studies of antivirals: Pihl, A. et al., Nature Scientific Reports volume 8, Article number: 17505 (2018) [Open Access]

In vitro anti-viral PK/PD

The hollow fibre culture system has been used to model human PK for anti-virals and to take samples for analysis over long periods of operation [more…]

FiberCell set up for virus production
C2011 cartridge module

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