Continous Production of Stem Cells

Continuous harvesting of OCT3/4 phentoype cells from a long term culture of human placenta-derived cells is possible using a FiberCell hollow fibre bioreactor.

When the harvested cells are transferred to flasks they differentiate into MSCs and generate spheroids.

Learn more : Application Note [PDF]

Phenotype of cells harvested directly from hollow fibre bioreactor compared to phenotype of bioreactor-harvested cells cultured in flasks

Phenotype:Cells from bioreactor:..followed by
3/5 days in Flask:
CD454%1%
CD340%0%
CD133/22%0%
CD313%48%
CD136%83%
CD10543%99%
CD7318%99%
CD905%96%

References:

Single-Use, Continuous Processing of Primary Stem Cells Whitford, W., Hardy, J., Cadwell, J.; BioProcessInternational 2014 12(3) [Download]

The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells: Cao, X et al.; Biotechnol. J. 2014, 9, 980-989 [Abstract]

Co-culture of Stromal and Erythroleukemia Cells in a Perfused Hollow Fiber Bioreactor System as an in vitro Bone Marrow Model for Myeloid Leukemia. Usuludin S, et al.; Biotechnol. Bioeng. 2012 109(5) [Open Access]

Starter kit for continuous stem cell processing R&D

Cat.#DescriptionQty
P3202FiberCell® Systems Duet1
C2008Low Flux PS Cartridge 5 kd (3000 cm2 – 20 ml)1
A – – –33mm, 38mm or 45mm Reservoir Cap1

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