Easy Scale-Up of Exosome/EVs Production
- Increase exosome yield by up to 100-fold compared to flask culture
- Obtain concentrated exosomes/EVs
- Serum-free high cell density bioreactor conditions
Standardizing production of large quantities of exosomes/ EV’s
A FiberCell hollow fibre bioreactor enables the standardized production of large amounts of ready-concentrated EVs from cells cultured or co-cultured at high density: tumour and engineered cell lines, stem cells etc.. Retained by the 20 kd MWCO fibres, EVs accumulate in the extra-capillary space and can be periodically harvested over a continuous culture period of weeks or even months.
Cells are not subjected to shear stress and are resistant to apoptosis in the perfused, capillarized cartridge resulting in clean EV harvests that are free of apoptotic bodies. Also the 20 Kd fibre cut-off blocks contamination from any bovine EVs due to serum in recirculating media.
To our knowledge, in hollow fibre culture, stem cells do not differentiate, resulting in linear production over time with the advantage of phenotypic stability.