Collect enriched secretome
Highly enriched conditioned media can be periodically collected, e.g. for secretome analysis / proteomics or to obtain large amounts of concentrated extracellular vesicles. FiberCell hollow fibre cartridges are available in a range of sizes with protein-retentive 5Kd or 20Kd molecular weight cut-offs. A special 0.1µm fibre is also available.
Under high cell density bioreactor conditions it is usually possible to completely replace serum with a chemically-defined substitute called CDM-HD that is protein-free and containing only low molecular weight components. In this way, a FiberCell bioreactor can further help the researcher to recapitulate in vivo growth conditions where cells are not exposed to serum.
Successful co-culture tissue modelling has been achieved using the FiberCell bioreactor, for example to create a bone marrow model or to investigate transendothelial migration of lymphocytes with endothelial cells grown as artificial capillaries, under flow conditions, on the luminal side of 0.1µm pore size fibres. Article: 3-D in vitroModels using Hollow Fiber Bioreactors