3D Culture of Cancer Cells

Many researchers are interested in 3D culture alternatives to standard flask culture because they are keen to better reproduce the physiological conditions under which cancer cells grow. In a hollow fibre bioreactor cells can grow post-confluently in 3 dimensions around perfused, porous “capillaries” resulting in very high cell densities of around 1E+8 cells/ml.

A complete FiberCell Hollow Fibre Bioreactor can be placed easily on the shelf of an incubator or inside a hypoxic chamber enabling the user to maintain more than 1E+9 cells. This means an enormous gain in convenience and efficiency in many situations.

Under high-density culture conditions serum can be replaced with a chemically-defined substitute from FiberCell Systems called CDM-HD that is free of proteins and contains only low molecular weight components. In this way, a FiberCell bioreactor helps to recapitulate in vivo growth conditions where cells are not usually exposed to serum.

Highly enriched conditioned media can be periodically collected, e.g. for secretome analysis / proteomics or collection of concentrated extracellular vesicles. FiberCell hollow fibre cartridges are available in a range of sizes with protein-retentive 5Kd or 20Kd molecular weight cut-offs. A special 0.1µm fibre is also available.

  • Secreted products 10-100X higher in concentration and in low volume
  • High density culture provides more physiologically relevant conditions
  • Serum-free, protein-free operation is usually possible (see CDM-HD)
Hollow fibre bioreactor fits in a standard incubator for convenient cell culture scale-up

A FiberCell® Bioreactor, complete with media reservoirs, will easily fit inside a standard incubator.

Starter kit for conditioned media production

Cat.# Description Qty
P3202 FiberCell® Systems Duet 1
C2011 High Flux PS Cartridge 20 kd (3000 cm2 – 20 ml)* 1
A – – – 33mm, 38mm or 45mm Reservoir Cap 1
CDM-HD Serum replacement 1

* Option: C2018 High Flux PS Cartridge 20 kd (1.2 m2 – 70 ml), providing 4 X more fibre surface area.

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