Collect enriched secretome
Highly enriched conditioned media can be periodically collected, e.g. for secretome analysis / proteomics or to obtain large amounts of concentrated extracellular vesicles. The culture of primary MSCs in the 20kDa FiberCell cartridge is a well-established application, for example. Protein- and EV-retentive 5Kd or 20Kd molecular weight cut-offs are available for polysulfone fibres. Special 0.1µm or 0.03µm microporous PVDF fibres are also available which may offer tighter protein matrix binding to enhance cell attachment.
Easily replace serum
Under high cell density bioreactor conditions that are naturally more supportive of growth it is usually possible to completely replace serum with a chemically-defined substitute called CDM-HD. This is a defined powder mix contains only low molecular weight components. A FiberCell bioreactor using CDM-HD media can further help the researcher to recapitulate in vivo growth conditions.
Successful co-culture tissue modelling has been achieved using the FiberCell bioreactor, for example to create a bone marrow model or to culture endothelial cells under flow conditions on the luminal side of 0.1µm pore size fibres. Article: 3-D in vitroModels using Hollow Fiber Bioreactors