Under high cell density bioreactor conditions it is usually possible to completely replace serum with a chemically-defined substitute called CDM-HD that is protein-free and containing only low molecular weight components. In this way, a FiberCell bioreactor can further help the researcher to recapitulate in vivo growth conditions where cells are not exposed to serum.
Collect enriched secretome
Highly enriched conditioned media can be periodically collected, e.g. for secretome analysis / proteomics or to obtain large amounts of concentrated extracellular vesicles. FiberCell hollow fibre cartridges are available in a range of sizes with protein-retentive 5Kd or 20Kd molecular weight cut-offs. A special 0.1µm fibre is also available.
Successful co-culture tissue modelling has been achieved using the FiberCell bioreactor, for example to create a bone marrow model or to investigate transendothelial migration of lymphocytes with endothelial cells grown as artificial capillaries, under flow conditions, on the luminal side of 0.1µm pore size fibres. Article: 3-D in vitroModels using Hollow Fiber Bioreactors