FiberCell Chemically Defined Media for High Density Cell Culture  – CDM-HD

Cultured in a FiberCell hollow fibre bioreactor, cells attain densities greater than 10e8/ml. At these high densities cell-secreted conditioning factors play a significant role in supporting the culture, to the extent that serum is no longer required. Serum can be completely replaced with a simple, low-cost chemically defined substitute, CDM-HD. No adaptation is required and CDM-HD can be employed once cells reach high density in the bioreactor. CDM-HD is protein-free and greatly facilitates product purification. It is also cGMP compliant.

Example uses of CDM-HD

MAb harvesting – 2 different MAbs

Secreted products such as monoclonal antibodies and recombinant proteins are free of contaminating proteins from the medium and can be purified using simplified protocols, increasing net yield in many cases.

Data courtesy of: Dr. Erin Bromage c/o the
US Veterinary Immune Reagents Network

Example use of CDM-HD

Antibody from the FiberCell HFBR was very clean and properly folded.

Lane 1: control (3 ug)
Lanes 2, 3 and 4: unpurified harvests from the cartridge on day 7, 9 and 12 (DMEM and 10% CDM-HD) (2µl)
Lane 5: 10X conc. supernatant from a T-flask (using DMEM and 10% CDM-HD) (2µl)
Lane 6: T flask (using DMEM and 10% serum) (2µl)

Concentration of supernatant from HFBR:  2.5 mg/ml.

Data courtesy of Liying Lu, University of Massachusetts, Worcester, Worcester, Massachusetts.

What are the components of CDM-HD serum substitute?

Components of CDM-HD: CDM-HD does not contain:
Customized ratios of amino acids and metabolites Protein
Vitamins and co-factors Hydrolysates
Trace elements Undefined reagents
Micro-nutrients Animal-derived components
Extra buffering capacity Steroid hormones or peptide mitogens
Recombinant growth factors Phenol red
Carbohydrates, including 1 gram of glucose/L Plurionic Detergent
All low molecular weight components, nothing larger than 3 kd High molecular weight materials including synthetic polymers

CDM-HD is produced according to ISO-1348

References:

Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag: Jayanthi S. et al.; Protein Expr Purif. 2014 Oct; 102: 76–84. [Open Access]

Take full advantage of high cell density mammalian cell culture
and avoid using serum

The inconvenience of serum-containing media and the high cost of specialty medias can be avoided by employing CDM-HD Serum Replacement in high density culture systems. Makes 10L protein-free media for around €250.

Product page, Cat.# etc.

Have a question?

Download the instructions for use or email us at info@kdbio.com