CDM-HD Serum Substitute

Serum replacement > makes 10 Litres serum-free media*

*Only for use in FiberCell® hollow fibre bioreactors or high density culture flasks.

  • Convenient – no adaptation period for hybridoma, CHO, HEK 293.
  • No proteins, No animal-derived components, No detergents
  • Highly consistent lot-to-lot, stable at 4°C for 1 month once made up
  • Avoids the typical risks of using serum (viruses, prions and mycoplasma)
  • Gives highest bioreactor yields

Easier purification

CDM-HD is a protein and surfactant-free serum substitute designed specifically for high density cell culture in the FiberCell® hollow fibre bioreactor. Using CDM-HD means simplified purification protocols and increased net yield.

How is it used?

CDM-HD is provided as a pack of dry powder to be made up to 1 litre and sterile-filtered to be used just like serum –  at a concentration of 10% in DMEM/High, for example. DMEM is the preferred basal media for highest cell density. One pack containing 33.36g enables 10 litres of serum-free media to be produced at low cost.

Optimized for Hollow Fibre Bioreactors

CDM-HD offers a serum substitute fully optimized to exploit the unique high density conditions in a FiberCell bioreactor. No adaptation period is needed: switch immediately to serum-free CDM-HD media once bioreactor glucose consumption reaches 1g/day.  CDM-HD contains no detergents or other cell membrane stabilizing agents that sometimes interfere with assays or precipitations [see composition table].


Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag: Jayanthi S. et al.; Protein Expr Purif. 2014 Oct; 102: 76–84. [Open Access]

Ordering Information

Cat. # Size Pack Size
CDM-HD Serum Substitute for High Density Cell Culture (enough to make 10 Litres media at 10% conc)    36 g

Request Quotation