This new article by John Cadwell, President and CEO of FiberCell Systems Inc., points out the specific advantages of hollow fibre bioreactors for monoclonal antibody production.
Hollow fibre systems provide cultured cells with a porous support matrix in place of the solid, impermeable plastic substrates that are used to produce 2D culture vessels. The molecular weight cut-off of the hollow fibre matrix retains hybridomas and antibodies but allows the inhibitory cytokine TGF-beta to diffuse away very efficiently thereby boosting productivity. A hollow fibre cartridge provides a capillarized, perfused, long term 3D culture system that enables ultra-high cell densities >10e8 cells/ml to be reached.
This means that with a FiberCell bioreactor you can easily maintain over 2 x 10e9 hybridoma cells on the shelf of a standard lab incubator to provide impressive amounts of concentrated antibody harvested during a period of one or two months (or even several months if needed).
See our applications page for examples and references here