Intestinal epithelial cells infected with Cryptosporidium parvum.
After 8 weeks of growth, the cartridge was cut open and slices of thin sections of the hollow fibers examined by Scanning Electron Microscopy. The intestinal epithelial cells had differentiated into crypt-villus units with microvilli (MV) covering them.
(A) Proximal end of the cartridge shows many craters (C) in the epithelial cell layer, typical of a C. parvum infection.
(B) Distal end of the cartridge has less epithelial damage and developmental stages are visible assacs covering the villi (DS).
Continuous culture of Cryptosporidium parvum using hollow fiber technology: Morada, M. et al.; Int J Parasitol. 2016 Jan;46(1):21-9 [related presentation PDF]