Easy Scale-Up of Exosome/EVs Production
- Increase exosome yield by around 100-fold compared to flask culture
- Obtain ready-concentrated exosomes/EVs
- No need for serum starvation prior to collection
Standardizing production of large quantities of exosomes/ EV’s
A FiberCell hollow fibre bioreactor enables the standardized production of large amounts of ready-concentrated exosomes/EVs from cells cultured or co-cultured at high density: tumour and engineered cell lines, stem cells etc.. Retained by the 20 kd MWCO fibres, exosomes accumulate in the extra-capillary space. They can be easily harvested every few days over a period of weeks or even months.
Cells are resistant to apoptosis in the perfused, capillarized cartridge resulting in clean exosome harvests that are free of apoptotic bodies. Also the fibre cut-off very effectively stops contamination by any bovine exosomes present in the recirculating media.
To our knowledge, in hollow fibre culture, stem cells do not differentiate, remaining phenotypically stable.