Scale-up Production of Conditioned Medium / Secretome Generation

FiberCell® Systems 20ml or 70 ml hollow fiber cell culture cartridges are ideal for the production of conditioned medium, EVs and generating the secretome from various cell types including tumor cell lines. Many billions of cells can be supported for weeks or, if required, months of continuous culture in the space of an incubator shelf.

  • Secreted products 10-100X higher in concentration and in low volume
  • High density culture provides more physiologically relevant conditions
  • Serum-free, protein-free operation may be possible (see CDM-HD)

Optimize your production run by choosing between a 5 kd or 20 kd molecular weight cut-off fibre.

Recent studies have shown that apoptosis is significantly reduced in the hollow fiber environment. The supernatant is free of intracellular proteins that might come from cell lysis.

Hollow fibre bioreactor fits in a standard incubator for convenient cell culture scale-up

A complete FiberCell® Systems Bioreactor for producing conditioned media will easily fit inside a standard incubator

Take advantage of HFBR conditions to make a switch to CDM-HD serum substitute. This way, conditioned medium and secretomes will be free from interfering serum and lytic proteins.

References:

Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker: Verathamjamras,C. et al; Int J Oncol. 2017 51(1):269-280. [Abstract]

Advances in the proteomic investigation of the cell secretome: Brown, K.J. et al; Expert Rev Proteomics. 2012 Jun; 9(3): 337–345. [Open access]

Effective enrichment of cholangiocarcinoma secretomes using the hollow fiber bioreactor culture system: Weeraphan, C.; Talanta. 2012 Sep 15;99:294-301 [Abstract]

Collection of in vivo-like liver cell secretome with alternative sample enrichment method using a hollow fiber bioreactor culture system combined with tangential flow filtration for secretomics analysis: Wen,Y-T et al.; Analytica Chimica Acta 2011 Vol. 684, p81-88 [Abstract]

Cell Secretome Analysis Using Hollow Fiber Culture System Leads to the Discovery of CLIC1 Protein as a Novel Plasma Marker for Nasopharangeal Carcinoma: Chang,YH et al.; J Proteome Res. 2009 8(12):5465-74. [Abstract]

Proteomics Analysis of Nasopharyngeal Carcinoma Cell Secretome Using a Hollow Fiber Culture System and Mass Spectrometry: Wu, HJ et al.; Journal of Proteome Research, 2009,8 (1), 380-389 [Open Access]

Starter kit for conditioned media production

Cat.# Description Qty
P3202 FiberCell® Systems Duet 1
C2011 High Flux PS Cartridge 20 kd (3000 cm2 – 20 ml)* 1
A – – – 33mm, 38mm or 45mm Reservoir Cap 1
CDM-HD Serum replacement 1

* Option: C2018 High Flux PS Cartridge 20 kd (1.2 m2 – 70 ml), providing 4 X more fibre surface area.

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